Purpose
Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
Inserts
SadCas9-KRAB
gRNA-TRE
Use
CRISPR and Lentiviral
Tags
HA
Expression
Mutation
gRNA sequence: ATCAGTGATAGAGAACGTATG
Available since
July 25, 2023
Availability
Academic Institutions and Nonprofits only