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pGEMT-GFP-p2a-aid

Plasmid

#161975

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GFP-aid-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCSHSP:FLP

Plasmid

#30521

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

FLP recombinase

Use

Flp/frt

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pFUW COX8ASig-mEmerald

Plasmid

#239557

Purpose

Expression and mitochondrial localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW COX8ASig-mCherry

Plasmid

#239558

Purpose

Expression and mitochondrial localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW LAMP1Sig-mEmerald

Plasmid

#239555

Purpose

Expression and lysosomal localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCCG_nit9fl_Gephyrin_linker

Plasmid

#228416

Purpose

Expression of NIT-9 with Gephyrin linkage region in N. crassa at the his-3 locus

Depositor

Robert Haensch

Insert

NIT-9 (GPHN Human)

Use

Protein expression in n. crassa

Mutation

removal of the linkage region and insertion of th…

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2S-C

Plasmid

#225967

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of serine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2S-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2N-C

Plasmid

#225968

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of asparagine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2N-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2D-C

Plasmid

#225969

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of aspartic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2D-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2E-C

Plasmid

#225970

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of glutamic acid in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2E-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2A-C

Plasmid

#225971

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGADTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTMCR-TLY-X2A-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2H-C

Plasmid

#225972

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGHDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2H-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2S-C

Plasmid

#225973

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGSDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2S-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2N-C

Plasmid

#225974

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGNDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2N-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2D-C

Plasmid

#225975

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGDDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2D-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2E-C

Plasmid

#225976

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2E-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2A-C

Plasmid

#225965

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of alanine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2A-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXWTM-X2H-C

Plasmid

#225966

Purpose

Block V Repeats-In-Toxin (RTX) protein with global substitution of histidine in nonapeptide repeats and C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXWTM-X2H-C

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD ATP6-2-1

Plasmid

#210829

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in ATP6-2 in mitochondria

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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mTALEAD nad7-2

Plasmid

#210832

Purpose

mTALEAD expression vector

Depositor

Shin-ichi Arimura

Article

Zhou et al Plant Physiol. 2023 Dec 21:kiad678. doi:

Insert

For specific A-to-G base editing to introduce targeted A-to-G mutation in nad7 in mitochondria

Expression

Plant

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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