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hsp70:mKate2-NWASP-CA

Plasmid

#105967

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/mNeptune2-nat

Plasmid

#120337

Purpose

Bacterial mNeptune2 labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Expression

Bacterial

Promoter

proD

Available Since

Aug. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-TTLL11

Plasmid

#140582

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA TTLL11

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD2-HA-LRR-NELC492A-C (B3-B4)

Plasmid

#211862

Purpose

Mutant E3-DART ligase in domestication vector

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

LRR-NEL

Use

Synthetic Biology

Tags

HA

Mutation

C492A

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-HEK3+24

Plasmid

#140577

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA HEK3 +24

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9aQ66L

Plasmid

#169072

Purpose

Express human constitutively active form of Rab9a(Q66L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q66L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-CLIC3

Plasmid

#140583

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CLIC3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD3-Lux-nat

Plasmid

#120323

Purpose

Bacterial luminescence promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Expression

Bacterial

Promoter

(None)

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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NK644

Plasmid

#109096

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_00306 geranyl-geranylation signal

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Available Since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-EMX1

Plasmid

#140581

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA EMX1

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pWT065c

Plasmid

#107913

Purpose

W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

(TCF-LEF)7-BE3-P2A-Luc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR15aPer

Plasmid

#21116

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
p5426

Plasmid

#168187

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::dsred PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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p3XFlag-YedK

Plasmid

#84567

Purpose

Expresses YedK in mammalian cells

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK

Tags

3xFlag and C-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-EMX1+38

Plasmid

#140579

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA EMX1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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p5204

Plasmid

#168181

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PEM7::dsred PcypD::sfgfp (frameshift)

Expression

Bacterial

Mutation

frameshift sfGFP

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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