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pBCC155

Plasmid

#74995

Purpose

CFP-based BiFC vector for transient expression of CC (155-238) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (155–239)/CC155

Expression

Plant

Promoter

CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVj-HACre

Plasmid

#78407

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
aur1R (aur1 Fission Yeast)

Expression

Yeast

Mutation

Gly 240 to Cys

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2049

Plasmid

#79657

Purpose

ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Publication

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple260-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2044

Plasmid

#79652

Purpose

ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Publication

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple255-EmGFP WPRE

Use

AAV

Promoter

PAX6 HS234Z Enhancer

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTU1-A-RFP

Plasmid

#72939

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBW412hrpL-cIgfp

Plasmid

#61438

Purpose

pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs32-CI-LVA-t-plam-30gfp-t

Tags

LVA

Expression

Bacterial

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pERB160

Plasmid

#58285

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Publication

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Tags

mCherry

Expression

Mammalian

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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psiCHECK-2-Zfp36-3'UTR

Plasmid

#61780

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36

Depositor

Judy Lieberman

Publication

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR

Use

Luciferase

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pJZC40

Plasmid

#62334

Purpose

sgRNA + 2x PP7 with mCherry for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2x PP7
mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pERB111

Plasmid

#58281

Purpose

constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Publication

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

empty miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Tags

GFP and mCherry

Expression

Mammalian

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJJH1260

Plasmid

#36913

Depositor

Jürgen Heinisch

Publication

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Inserts

ScURA3
ScURA3

Expression

Bacterial, Mammalian, and Y…

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-rtTA3G-TetOn3G

Plasmid

#250672

Purpose

Drive Tet-ON activator expression from human synapsin promoter for doxycycline-inducible transgene activation

Depositor

Changyang Linghu

Publication

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

rtTA3G-TetOn3G

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-DIO-Lck-mCherry-ibARK-4x6T

Plasmid

#241243

Purpose

Cre-dependent expression plasma membrane-tethered ibARK with higher specificity in astrocytes

Depositor

Jun Nagai

Publication

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

DIO-Lck-mCherry-ibARK-4x6T

Use

AAV

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

Plasmid

#244204

Purpose

Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SpCas9D10A nickase

Plasmid

#216737

Purpose

Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

Depositor

Vincent Dion

Publication

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase

Use

AAV and CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

CMVd1

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pQFT-mNG-Pcon-Scar

Plasmid

#203678

Purpose

pQFT-mNG with constitutively expressed mScarlet-I

Depositor

Urs Jenal

Publication

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
mScarlet-I

Expression

Bacterial

Promoter

PQJ and Phyb18

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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SPDK3959

Plasmid

#199246

Purpose

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

Depositor

Savithramma Dinesh-Kumar

Publication

Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

Insert

Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Promoter

PEBV

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Publication

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-UBC-EGFP-2A-BoNT-B (LC)

Plasmid

#185722

Purpose

Lentiviral expression of GFP and botulinum neurotoxin type B light chain from UbC promoter

Depositor

Jun Ding

Publication

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

botulinum neurotoxin type B light chain

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-TdTomato

Plasmid

#192658

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, TRE3G

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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