169,724 results
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Plasmid#133966PurposeExpresses BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE3
UseCRISPR and Synthetic BiologyAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_TurboID_FLAG_Nt
Plasmid#194072Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-TurboID-FLAGExpressionMammalianAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiFLAGscFv-TM
Plasmid#198303PurposeDisplays anti-FLAG scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiFLAGscFv-TM
ExpressionMammalianAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP1-Flag-His-7xKQ
Plasmid#111573PurposeExpresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cellsDepositorInsertPARP1 (PARP1 Human)
TagsC-terminal 6xHis and C-terminal FlagExpressionInsectMutationlysine to glutamine mutation at the following ami…Promoterpolyhedrin promoterAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode
Plasmid#170240PurposeBarcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3231
Plasmid#144707PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2905
Plasmid#143660PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2488
Plasmid#142432PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b FAST-PETase
Plasmid#225760PurposeExpresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tagDepositorInsertFAST-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only