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EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
SnapTag-Binder

Plasmid

#179142

Purpose

Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

SspB

Tags

SNAP-tag

Expression

Mammalian

Mutation

Y11K/A15E, residue numbering from pdb: 1ou9

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

Plasmid

#108735

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

Plasmid

#35500

Purpose

AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

ChR1-VChR1 Chimera

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E122T and E162T

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shcGAS-Mmu

Plasmid

#127698

Purpose

Doxycyclin inducible shRNA knockdown of mouse cGAS gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shcGAS Mmu (Cgas Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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TK-GFP-Nluc-P2A-neo-TK

Plasmid

#134896

Purpose

Expresses GFP protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum TK locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Insert

TK-GFP-Nluc-P2A-Neo-TK

Use

Cryptosporidium expression

Promoter

Cryptosporidium actin and enolase

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-EEA1

Plasmid

#109560

Purpose

Multisite gateway vector for 5' tagging with early endosome associated 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

early endosome associated 1 (eea1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5a-Myc

Plasmid

#186613

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5A (KIF5A Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Promoter

hU6

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-G78)-FRB

Plasmid

#154447

Purpose

expresses pBirA(1-G78)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-G78)-FRB

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108770

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-EFIa-Id1-PGK-puro

Plasmid

#107735

Purpose

Expresses Id1 in mammalian cells

Depositor

Alexandre Colas

Article

Cunningham et al Genes Dev. 2017 Aug 9. pii: gad.300400.1

Insert

Id1 (T Mouse)

Use

Lentiviral

Promoter

EF1a

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

Plasmid

#108737

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA

Plasmid

#108729

Purpose

Expresses N-terminus to aa270 of Cre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa270

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-WT

Plasmid

#101048

Purpose

Expresses HA-tagged human Rab8-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-hNF2

Plasmid

#107150

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2-mCherry

Plasmid

#140571

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA

Plasmid

#108738

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-mCherry-Binder

Plasmid

#179139

Purpose

Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

SspB

Tags

FLAG and mCherry

Expression

Mammalian

Mutation

Y11K/A15E, residue numbering from pdb: 1ou9

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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rag2:mcherry_CE_pISceI

Plasmid

#223047

Purpose

Express m-Cherry gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

m-Cherry

Use

Express the insert(gene) in mesenchymal lineage o…

Promoter

Rag2

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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