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pSMVP-Cas9N

Plasmid

#80934

Purpose

Plasmid that expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAL-pck_NT

Plasmid

#74069

Purpose

pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (NT)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
LP538

Plasmid

#90202

Purpose

pCMV-myc with cDNA encoding HsTRAPPC8 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Tags

Myc

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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PSICORhp-mp53

Plasmid

#26273

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p53 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pFtnA-Halo

Plasmid

#98281

Purpose

mammalian expression of FtnA-Halo

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Tags

Halo-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9C

Plasmid

#80939

Purpose

Plasmid that expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET-51b-HaloTag10

Plasmid

#175520

Purpose

Expression of HaloTag10 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag10

Tags

His

Expression

Bacterial

Mutation

HaloTag10 = HaloTag7-Q165W

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGT-Ah3

Plasmid

#122570

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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rOpto-rtrkB_397

Plasmid

#82681

Purpose

Expresses rOpto-rtrkB (light-activated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

Insert

rOpto-rtrkB

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pNC-Green-LUC

Plasmid

#193438

Purpose

Nimble cloning-compatible vector for the analysis of promoter activity in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-mRFP-GFPnb

Plasmid

#113555

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-mRFP-GFPnb

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ141B-ge4.1

Plasmid

#205144

Purpose

Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

Depositor

Yiping Qi

Article

Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Promoter

AtU3

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3

Plasmid

#203461

Purpose

Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway entry vector

Tags

Mitochondrial localization signal

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Magenta

Plasmid

#160446

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Expression

Worm

Promoter

EFT3

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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