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pMuLE ENTR CMV CreERT2 R4-R3

Plasmid

#62173

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

CreERT2

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR eGFP L5-L2

Plasmid

#62183

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR-U6gRNA-PGKpuro2ABFP

Plasmid

#136405

Purpose

Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Type

Empty backbone

Use

CRISPR

Mutation

BbsI restriction site at position 437 was removed…

Promoter

U6, PDK

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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AA015

Plasmid

#216058

Purpose

Fragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFP

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA406

Plasmid

#216070

Purpose

Fragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistance

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

P2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-LMP2A

Plasmid

#195816

Purpose

Gateway Entry Clone of Epstein-Barr virus LMP2A

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

LMP2A (LMP-2A Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2a

Plasmid

#195767

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2a

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2a (BNLF2a Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2b

Plasmid

#195770

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2b

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2b (BNLF2b Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pQTEV-FBXO31

Plasmid

#34815

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

F-box protein 31 (FBXO31 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pQStrep2-GADD45G

Plasmid

#31584

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

growth arrest and DNA-damage-inducible, gamma (GADD45G Human)

Tags

His and Strep

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 CreERT2 L3-L2

Plasmid

#62174

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

CreERT2

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB527

Plasmid

#74869

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV LacZ L5-L2

Plasmid

#62166

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

LacZ

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB525

Plasmid

#74867

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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MK13_HUMAN_D0

Plasmid

#79693

Purpose

This plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK13

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PAK7_HUMAN_D0

Plasmid

#79706

Purpose

This plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK7 (PAK5 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCR-Blunt II-TOPO-PTPRD

Plasmid

#29705

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta (PTPRD Human)

Use

Cloning vector

Mutation

none

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pGWB429

Plasmid

#74823

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 mCherry L3-L2

Plasmid

#62150

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-5

Plasmid

#184017

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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