We narrowed to 62,227 results for: cloning

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pME mKate2-dynactin1-811

Plasmid

#105971

Purpose

entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

dynactin1-811 (DCTN1 Human)

Tags

mKate2

Expression

Bacterial

Mutation

contains only amino acids 1-811

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pM.010

Plasmid

#228696

Purpose

C terminal Strep affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

TEV-Strep

Expression

Insect

Mutation

WT

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pM.009

Plasmid

#228695

Purpose

N terminal Strep affinity tag with TEV cleavage site

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

Strep-TEV

Expression

Insect

Mutation

WT

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1e

Plasmid

#228679

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1d

Plasmid

#228678

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1c

Plasmid

#228677

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1b

Plasmid

#228676

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1a

Plasmid

#228675

Purpose

Moclo Baculo toolkit level 2 backbone (biGBac compatible)

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Type

Empty backbone

Expression

Insect

Mutation

GentR R98K

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.007

Plasmid

#228673

Purpose

tSV40 Terminator for Baculovirus expression without Cterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

tSV40

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB.005

Plasmid

#228671

Purpose

polH Promoter for Baculovirus expression without Nterminal tag

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

polH

Expression

Insect

Mutation

WT

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGGEV_4_XcmI-LacZ

Plasmid

#49299

Purpose

Golden Gate entry vector at position 4, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_5_XcmI-LacZ

Plasmid

#49300

Purpose

Golden Gate entry vector at position 5, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_6_XcmI-LacZ

Plasmid

#49301

Purpose

Golden Gate entry vector at position 6, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_7_XcmI-LacZ

Plasmid

#49302

Purpose

Golden Gate entry vector at position 7, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frC

Plasmid

#253184

Purpose

For recombinant virus rescue; encodes fragment C of SARS-CoV-2 (nt15100-20958)

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frC

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_SV40

Plasmid

#253185

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867)

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frB

Plasmid

#253183

Purpose

For recombinant virus rescue; encodes fragment B of SARS-CoV-2 (nt8590-15107)

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frB

Expression

Bacterial

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pYSD080

Plasmid

#212917

Purpose

Encodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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