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pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)

Plasmid

#44094

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

2xStrep tag, 6xGly linker, ADH1 transcription ter…

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-Purg1

Plasmid

#39350

Depositor

Jurg Bahler

Publication

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

natMX6 module - reverse orientation

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1-sh-mSlug-3

Plasmid

#40647

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-3 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
TAL2289

Plasmid

#36724

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCG-TALEN_Right (Fancg Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLKO-puro-sh-mFAK B

Plasmid

#37018

Depositor

Bob Weinberg

Publication

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

sh-mFAK B (Ptk2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

May 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Publication

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
CD200Cd4d3+4-bio

Plasmid

#32404

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Publication

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBP-mTAZ-NFLAG

Plasmid

#31791

Depositor

Michael Yaffe

Publication

Hong et al Science. 2005 Aug 12;309(5737):1074-8.

Insert

TAZ (Wwtr1 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEMS1052

Plasmid

#29004

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple112 (HCRT Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Cre-EGFP-NLS

Available since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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416Gal-FUS-R521C-YFP

Plasmid

#29622

Depositor

Aaron Gitler

Publication

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R521C

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
TRMPV-ns

Plasmid

#27991

Purpose

Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.

Depositor

Scott Lowe

Publication

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPV-ns (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Expression

Mammalian

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Publication

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Tags

GFP

Expression

Worm

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMP220

Plasmid

#185921

Purpose

Broad host range IncP promoter probe using lacZ

Depositor

Herman Spaink

Publication

Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi

Type

Empty backbone

Use

Incp

Tags

LacZ

Expression

Bacterial

Promoter

None

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

Plasmid

#185802

Purpose

one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

Depositor

Robin Lee

Publication

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag

Expression

Mammalian

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

Plasmid

#174030

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-Venus-PEST

Use

Zebrafish knock-in tagging

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pWVR5

Plasmid

#167509

Purpose

Broad-Host range expression vector, pSa origin, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFNR5

Plasmid

#149483

Purpose

Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBNG2

Plasmid

#149473

Purpose

Broad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Publication

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJS102

Plasmid

#118281

Purpose

Expresses GFPmut2 with low cell-to-cell variation and lactose induction

Depositor

Zach Hensel

Publication

Silva et al mSphere. 2019 May 29;4(3). pii: 4/3/e003

Insert

GFPmut2

Expression

Bacterial

Promoter

pLlacO-1

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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