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GIV-FLAG L249P (LP)

Plasmid

#86097

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Article

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

L249P

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS2025

Plasmid

#49132

Purpose

AAV plasmid with NOV (Ple303) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple303-iCre

Use

AAV

Expression

Mammalian

Promoter

NOV

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pFOSGE_COF3H04

Plasmid

#70971

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf6 (Taf6 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C07

Plasmid

#70985

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

14-3-3zeta (14-3-3zeta Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pLPC-NMYC-mTRF1deltaBLM

Plasmid

#64163

Purpose

Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted amino acids 313-316 and 339-342 (both BLM…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pGMCK-0X750-luxAB-DAS+4

Plasmid

#49517

Purpose

Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-M100/125/147L-siResist

Plasmid

#49856

Purpose

Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, and Methionine 147…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
GoldyTALEN-ppp1cab TAL2

Plasmid

#41836

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

protein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)

Use

Cloning vector

Available Since

Feb. 1, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-moesina TAL1

Plasmid

#41831

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

moesin a (msna Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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GoldyTALEN-moesina TAL2

Plasmid

#41832

Depositor

Jeffrey Essner

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

moesin a (msna Zebrafish)

Use

Cloning vector

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAMTS18 (P1035S)

Plasmid

#29558

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 1035 to serine

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (Q1002X)

Plasmid

#29557

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed glutamine 1002 to stop codon

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (Q904X)

Plasmid

#29556

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed glutamine 904 to stop codon

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (C638S)

Plasmid

#29555

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed cysteine 638 to serine

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ADAMTS18 (G312E)

Plasmid

#29553

Depositor

Yardena Samuels

Article

Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

Insert

ADAMTS18 (ADAMTS18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed glycine 312 to glutamic acid

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Mouse, Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBwt-PURO

Plasmid

#208045

Purpose

Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

Ub (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEVOL-pAzF[TAG]

Plasmid

#164579

Purpose

Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

araBAD, glnS, and proK

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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