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pHCas9-Nours

Plasmid

#107733

Purpose

Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

Depositor

Sheng Yang

Article

Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Human optimized

Promoter

TEF1

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTG-mTDGa.1

Plasmid

#81049

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMTgR2

Plasmid

#107286

Purpose

Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

Depositor

Bohdan Schneider

Article

Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

Insert

Interferon gamma receptor 2 (IFNGR2 Human)

Tags

CATCATCACCATCACCATGA

Expression

Insect

Promoter

Metallothionein promoter

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGBT9-chNfasc (+RSLE)

Plasmid

#89418

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

NFASC (NFASC Chicken)

Tags

GAL4 DBD

Expression

Yeast

Promoter

ADH1

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGBT9-hSyntenin2a

Plasmid

#89421

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Tags

GAL4 DBD

Expression

Yeast

Promoter

ADH1

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway Cloning

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3f(S, +)-AP-His

Plasmid

#72023

Purpose

Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains no deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(L)-AP-His

Plasmid

#72025

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.y-AP-His

Plasmid

#72055

Purpose

Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.y (Unc5c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5d.z-Fc-His

Plasmid

#72185

Purpose

Expresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.z (Unc5d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.y-Fc-His

Plasmid

#72181

Purpose

Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.y (Unc5c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.z-Fc-His

Plasmid

#72182

Purpose

Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.z (Unc5c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn3-AP-His

Plasmid

#71979

Purpose

Expresses the entire Netrin 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn3 (Ntn3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb3-AP-His

Plasmid

#72004

Purpose

Expresses the extracellular region of the PlexinB3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb3 (Plxnb3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Plxnb2(L)-AP-His

Plasmid

#72002

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Dectin1A-C-10

Plasmid

#55413

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Dectin1A-N-10

Plasmid

#55414

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (R544W)

Plasmid

#29540

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E452K)

Plasmid

#29538

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only