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Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RNaseHI-NES-EGFP
Plasmid#196701PurposePlasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.DepositorInsertbacterial RNaseHI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-GFP
Plasmid#129092PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertEGFP and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-CreER
Plasmid#129093PurposeKI CreER with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertCreERT2 and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.006
Plasmid#119670PurposeLevel 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.DepositorInsertPcpc560-Ux2
UseSynthetic BiologyTagsExpressionMutationbp 89 T to C, bp 187 C to A, Duplicated upstream …PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
Plasmid#196703PurposePlasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.DepositorInserthuman RNaseH1 D210N
UseTagsExpressionMammalianMutationD210N, catalytically inactivePromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
APJ-DuET
Plasmid#213185PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertAPJ (APLNR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
APJ-Tango
Plasmid#66224PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertAPJ (APLNR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-iCT
Plasmid#129094PurposeKI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertiCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-a
Plasmid#108538PurposeExpresses human PCDH7 transcript variant a in mammalian cellsDepositorInsertPCDH7 transcript variant a (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CTLA4-FLN-ELP-His-ybbr
Plasmid#168038PurposeExpresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertCytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
UseTags3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX303-PCDH7-d
Plasmid#108541PurposeExpresses human PCDH7 transcript variant d in mammalian cellsDepositorInsertPCDH7 transcript variant d (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-PCDH7-c
Plasmid#108540PurposeExpresses human PCDH7 transcript variant c in mammalian cellsDepositorInsertPCDH7 transcript variant c (PCDH7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only