We narrowed to 2,123 results for: PAM

1. pYPQAT30

   Plasmid

   #223402

   Purpose

   T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQAT14

   Plasmid

   #223386

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p35S::Cf-4-YFPn

   Plasmid

   #86161

   Purpose

   Cf-4 split YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   Cf-4
   

   Tags

   YFPn

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p35S::Cf-4-YFPc

   Plasmid

   #86160

   Purpose

   Cf-4 slplit YFP

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   Cf-4
   

   Tags

   YFPc

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. xCas9

   Plasmid

   #138565

   Purpose

   Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   xCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEE901-A3A

   Plasmid

   #229044

   Purpose

   nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p35S::AtSOBIR1-mCherry

   Plasmid

   #86166

   Purpose

   SOBIR1-mCherry expression

   Depositor

   Silke Robatzek

   Article

   Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

   Insert

   SOBIR1 (SOBIR1 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA299

    Plasmid

    #216029

    Purpose

    Fragmid fragment: (Cas protein) NNGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Slug]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA296

    Plasmid

    #216028

    Purpose

    Fragmid fragment: (Cas protein) NNNNCC PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Nme2Cas9_v1.1 [Nme2]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA301

    Plasmid

    #216030

    Purpose

    Fragmid fragment: (Cas protein) NNGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sauri]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTBL437 AAVS1 EQR sgRNA

    Plasmid

    #126447

    Purpose

    To cut the human AAVS1 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human AAVS1 safe-harbor locus with NGAG PAM
    

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. F11-R pEBio

    Plasmid

    #61486

    Purpose

    Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    F11R (F11R Human)
    

    Tags

    enzymatic biotinylation sequence and rat CD4 d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. XLone-Fully-spliced CRNDE

    Plasmid

    #242178

    Purpose

    Express doxycycline inducible Fully-spliced CRNDE in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

    Insert

    Fully-spliced CRNDE (CRNDE Human)
    

    Expression

    Mammalian

    Mutation

    PAM mutation (CCT to GTT)

    Promoter

    TRE3GS

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. XLone-Unspliced CRNDE

    Plasmid

    #242177

    Purpose

    Express doxycycline inducible Unspliced CRNDE in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

    Insert

    Unspliced CRNDE (CRNDE Human)
    

    Expression

    Mammalian

    Mutation

    PAM mutation (CCT to GTT)

    Promoter

    TRE3GS

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSIP103: pTarget(CyCAST)

    Plasmid

    #200849

    Purpose

    Target plasmid containing CyPSP1 and attachement site for CyCAST.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Inserts

    PAM for CyCAST + CyPSP1
    tRNA-Leu
    

    Expression

    Bacterial

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTBL436 HEK293site3EQR sgRNA

    Plasmid

    #126445

    Purpose

    To cut the human HEK293 site3 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human HEK293 site3 with NGAG PAM
    

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p35S::SISOBIR1-YFPc

    Plasmid

    #86164

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Tags

    YFPc

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p35S::AtSOBIR1-YFPn

    Plasmid

    #86165

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1 (SOBIR1 Mustard Weed)
    

    Tags

    YFPn

    Expression

    Plant

    Promoter

    35S

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge