We narrowed to 1,044 results for: Psp;

1. pTRE/SP S245X with 5'UTR

   Plasmid

   #92376

   Purpose

   Inducible expression of S245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE/SP M87 S245X

   Plasmid

   #92374

   Purpose

   Inducible expression of M87 S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, S245X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE/SP M1 S245X

   Plasmid

   #92372

   Purpose

   Inducible expression of M1S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, S245X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE/SP M1 N184X

   Plasmid

   #92371

   Purpose

   Inducible expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   Tet-responsive P tight

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Tag/SP S245X with 5'UTR

   Plasmid

   #92370

   Purpose

   expression of 245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-Tag/M87 N184X

   Plasmid

   #92367

   Purpose

   expression of M87 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, N184X

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Tag/M1 N184X

   Plasmid

   #92365

   Purpose

   expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   CMV

   Available Since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Tag 3B/myc-M87L195V

   Plasmid

   #87721

   Purpose

   Expresses myc-M87L195V spastin isoform in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   M87 isoform (deletion of nt 1-490 - 5'UTR an…

   Promoter

   CMV

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-Tag/S46X without 5'UTR

   Plasmid

   #87730

   Purpose

   Expresses S46X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

   Insert

   SPAST (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   S46X; 5'UTR deleted (1-190 bp)

   Promoter

   CMV

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Tag/S44L without 5'UTR

    Plasmid

    #87728

    Purpose

    Expresses S44L spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    S44L; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Tag/L195V without 5'UTR

    Plasmid

    #87726

    Purpose

    Expresses L195V spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    L195V; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Tag/E112K without 5'UTR

    Plasmid

    #87724

    Purpose

    Expresses E112K spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    E112K; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Tag 3B/myc-M87E112K

    Plasmid

    #87720

    Purpose

    Expresses myc-M87E112K spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mApple-M1 Spastin

    Plasmid

    #134461

    Purpose

    M1 Spastin expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    Met to Ala change at amino acid 87

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMGS7 (AAVS1sgRNA)

    Plasmid

    #126582

    Purpose

    A CRISPR/Cas9 construct to target the AAVS1 locus in humans

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AAVS1 sgRNA
    

    Expression

    Mammalian

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-Ef1a-Cas9-H2B-GFP

    Plasmid

    #159654

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRE-VEGFA EF1a-rtTA3

    Plasmid

    #195881

    Purpose

    Lentiviral construct of doxycycline inducible VEGFA

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    VEGFA (Vegfa Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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