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pET GFP domain 11 LIC cloning vector (GFP C-11)

Plasmid

#30183

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis, AviTag (biotin), GFP domain 11, and TEV cl…

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 ProteinG TEV LIC cloning vector (2P-T)

Plasmid

#29713

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-ProteinG-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

Plasmid

#60649

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pET PPL His6 MBP LIC cloning vector (2K-T)

Plasmid

#37183

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6, MBP, and PPL

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Anti-GluN3A/NR3A glutamate receptor [N416/57R]

Plasmid

#206618

Purpose

Mammalian Expression Plasmid of anti-GluN3A/NR3A glutamate receptor (Mouse). Derived from hybridoma N416/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GluN3A/NR3A glutamate receptor (Mus musculus) recombinant Mouse monoclonal antibody (Grin3a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

Plasmid

#114427

Purpose

Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

PGK, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET Trx His6 LIC cloning vector (2Tc-T)

Plasmid

#37239

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis and Thioredoxin (Trx)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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Anti-GluA2/GluR2 glutamate receptor [L21/32R-2b]

Plasmid

#199405

Purpose

Mammalian expression plasmid of Anti-GluA2/GluR2 glutamate receptor [L21/32R-2b] (Rat). Derived from hybridoma L21/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GluA2/GluR2 glutamate receptor (Rat) recombinant mouse monoclonal antibody (Gria2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-GluN2B/NR2B glutamate receptor [N59/36.1R]

Plasmid

#114508

Purpose

Mammalian Expression Plasmid of anti-GluN2B/NR2B glutamate receptor (Rat). Derived from hybridoma N59/36.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grin2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

Plasmid

#136270

Purpose

Expresses ABE and ST2-com modified sgRNA scaffold

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Insert

ABE and sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Cre-responsive Cas9 expressing transposon library vector

Plasmid

#164138

Purpose

Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6, EF-1a

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-mGluR1/5 (Group I) glutamate receptor [N75/33R]

Plasmid

#177570

Purpose

Mammalian Expression Plasmid of anti-mGluR1/5 (Group I) glutamate receptor (Rat). Derived from hybridoma N75/33.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-mGluR1/5 (Group I) glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody (Grm5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 prescission LIC cloning vector (HPKS)

Plasmid

#29720

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

6xHis and Prescission cleavage site

Expression

Bacterial

Mutation

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Ai170 (TIT2L-ASAP2s-Kv-ICL-tTA2) targeting vector

Plasmid

#114435

Purpose

Target a Cre-dependent voltage sensor ASAP2s cassette into the mouse TIGRE locus

Depositor

Michael Lin, Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

ASAP2s-Kv, tTA2

Use

Cre/Lox and Mouse Targeting

Tags

Kv2.1 tag

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET mOCR His6 LIC cloning vector (2Oc-T)

Plasmid

#37238

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and mOCR

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV co-transformation cloning vector (13S-B)

Plasmid

#48324

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

Plasmid

#132560

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop replaced by com aptamer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV co-transformation cloning vector (13K-B)

Plasmid

#48316

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

Plasmid

#114429

Purpose

Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GFP

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV co-transformation cloning vector (13S-R)

Plasmid

#48328

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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