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pMV_AA089

Plasmid

#216105

Purpose

gRNA expression for Cas12a (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRWJB006

Plasmid

#160113

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

35S

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRWJB004

Plasmid

#160112

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Plant

Mutation

Promoter

UBQ10

Available since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGW-FL-V5

Plasmid

#78858

Purpose

DULIP Prey Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-RL-PA

Plasmid

#78856

Purpose

DULIP Bait Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Renilla luciferase, Protein A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLX-B3omega1 (GB1694)

Plasmid

#160643

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

Depositor

Fabio Pasin, Juan Antonio García

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Fabio Pasin, Juan Antonio García

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-B3omega2 (GB1695)

Plasmid

#160644

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)

Depositor

Fabio Pasin, Juan Antonio García

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-B2alpha2 (GB1696)

Plasmid

#160620

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)

Depositor

Fabio Pasin, Juan Antonio García

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-eGFP-HF

Plasmid

#157735

Purpose

mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

eGFP-HF

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCGN H-Ras 61L

Plasmid

#14720

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

H-ras (HRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

61L

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only
pBS-SKII-URA-GAL1-fCLIP-GS

Plasmid

#188939

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-GS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-GS

Plasmid

#188938

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-GS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-3xHA

Plasmid

#188935

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-3xHA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-fCLIP-3xHA

Plasmid

#188936

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-3xHA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-HALO-GS

Plasmid

#188940

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

pBS-SKII-URA-GAL1-HALO-GS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pIOM14

Plasmid

#20868

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aacC1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pBS-SKII-URA-GAL1-DHFR-3xHA

Plasmid

#188934

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-DHFR-3xHA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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E[beta]P

Plasmid

#24313

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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