We narrowed to 2,617 results for: pCas

Showing: 881 - 900 of 2617 results

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Publication

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCasper-pNos-PCP-eGFP

Plasmid

#180274

Purpose

to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Publication

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

PCP-eGFP

Tags

eGFP

Expression

Insect

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMS24: pCascade(PmcCAST)_entry

Plasmid

#168157

Purpose

Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS7: pCascade(AvCAST)_entry

Plasmid

#168140

Purpose

Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Publication

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-HilA empty vector

Plasmid

#153333

Purpose

Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

Depositor

Josef Penninger

Publication

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Type

Empty backbone

Use

Salmonella expression

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-NS1-HilA

Plasmid

#153327

Purpose

Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Publication

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-NS1 Monobody

Use

Salmonella expression

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Publication

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gamma-tubulin sgRNA

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSpCas9(BB)-2A-Puro-Exon4-PAFAH1B1

Plasmid

#92433

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Ralf Brandes, Matthias Leisegang

Publication

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Expression

Mammalian

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HeFSpCas9-H840A

Plasmid

#80456

Purpose

high fidelity enhanced SpCas9 mammalian expression vector

Depositor

Ervin Welker

Insert

HeFSpCas9_H840A

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CBh

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCaSpeR-4 porcupine (genomic DNA)

Plasmid

#37376

Depositor

Norbert Perrimon

Publication

Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

Insert

porcupine (por Fly)

Expression

Insect

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Publication

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

Plasmid

#113162

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

Depositor

Brandon Harvey

Publication

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Tags

FLAG

Expression

Mammalian

Mutation

D10A

Promoter

CAG

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)

Plasmid

#197499

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…

Promoter

CMV and T7

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

Plasmid

#197501

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

Promoter

CMV and T7

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

Plasmid

#197500

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

Promoter

CMV and T7

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Publication

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

Plasmid

#132560

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Publication

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

ABEmax
sgRNA, with Tetraloop replaced by com aptamer

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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