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We narrowed to 967 results for: plasmids spcas9

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Showing: 881 - 900 of 967 results

  3. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    131312-plasmid-map-sequence-id-254654

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  7. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    107269-plasmid-map-sequence-id-208617

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  9. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131300-plasmid-map-sequence-id-254651

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  11. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid
    #234724
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
    Depositor
    Kirill Martemyanov
    Article
    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234724-plasmid-map-sequence-id-466565

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  12. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Article
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Promoter
    T3 promoter
    Available Since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

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  13. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Agata Smogorzewska
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    99154-plasmid-map-sequence-id-352449

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  15. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  17. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid
    #140004
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140004-plasmid-map-sequence-id-275398

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  19. BE3-hA3A-R128A

    Plasmid
    #132944
    Purpose
    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-hA3A(R128A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    BE3-hA3A(R128A)
    Available Since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132944-plasmid-map-sequence-id-314824

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Showing: 881 - 900 of 967 results