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AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163475

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP1038-pAAV-mscRE16-minBGpromoter-FlpO-WPRE-hGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-YAP

Plasmid

#174168

Purpose

Lentiviral vector to constitutively express YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_KRAS_p.G12C

Plasmid

#81667

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PGKp-GFP-TAZ

Plasmid

#174176

Purpose

Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_SMARCB1_WT

Plasmid

#81791

Purpose

Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMARCB1 (SMARCB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132H

Plasmid

#81686

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

R132H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK1_WT

Plasmid

#82145

Purpose

Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK1 (MAPK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-BRAT1

Plasmid

#161920

Purpose

Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

BRCA1-associated ATM activator 1 (BRAT1 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_FGF1_WT

Plasmid

#81825

Purpose

Gateway Donor vector containing FGF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FGF1 (FGF1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163480

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12V

Plasmid

#81665

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132C

Plasmid

#81726

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway entry vector

Mutation

R132C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP7

Plasmid

#158759

Purpose

Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_WT

Plasmid

#81778

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

Plasmid

#179905

Purpose

Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

Insert

SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_TCF4_WT

Plasmid

#82166

Purpose

Gateway Donor vector containing TCF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TCF4 (TCF4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-EWSR1 Stop

Plasmid

#84887

Purpose

DONOR vector for Gateway cloning of EWSR1

Depositor

Aaron Gitler

Article

Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

Insert

EWSR1 (EWSR1 Human)

Use

Gateway donor

Promoter

none

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_FURIN_WT

Plasmid

#82122

Purpose

Gateway Donor vector containing FURIN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FURIN (FURIN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge