We narrowed to 26,138 results for: Vit

1. pDARMO_CMVT_3xFLAG-HUWE1_delta169-189

   Plasmid

   #187135

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 delta169-189

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   delta169-189

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951

   Plasmid

   #187133

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   delta3896-3951

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

   Plasmid

   #187129

   Purpose

   mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   I3969A/F3982A

   Promoter

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-HUWE1_UBA/UIM/UBM-mut

   Plasmid

   #187153

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA251_ Ptrc.x.tetO2::D-HicDH

   Plasmid

   #185456

   Purpose

   CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   D-HicDH from Lactobacillus paracasei DSM 20008
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc.x.tetO2

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUGW-shRIIα

   Plasmid

   #183454

   Purpose

   sgRNA targeting rat PKA-RIIα subunits

   Depositor

   Matthew Gold

   Article

   Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

   Insert

   sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   shRNA: H1 / gene: ubiquitin

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT079

   Plasmid

   #180509

   Purpose

   Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAT15541_dCBE-SuperFi-Cas9

   Plasmid

   #184371

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dCBE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT15543_dABE-SuperFi-Cas9

   Plasmid

   #184373

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT2/CAGXSP/PalmGamillus

    Plasmid

    #182971

    Purpose

    Stably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    PalmGamillus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. HEpl016

    Plasmid

    #179991

    Purpose

    pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    SiCas12i
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmMextli-M605D_AB

    Plasmid

    #148344

    Purpose

    Insect Expression of DmMextli-M605D

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    DmMextli-M605D (mxt Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    4 silent mutations compared to the sequence given…

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_AB

    Plasmid

    #148357

    Purpose

    Bacterial Expression of DmMextli_577-620-I583RS590R

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    DmMextli_577-620-I583RS590R (mxt Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FLAG-APEX2-GABARAPL2

    Plasmid

    #175751

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

    Plasmid

    #175752

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y49A L50A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP72-RBM8A-3'UTR AluY-Sz mut 2598-4066

    Plasmid

    #175586

    Purpose

    in vitro transcription of mut RBM8A 3'UTR

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A-3'UTR AluY-Sz mut 2598-4066
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330 NLC-FKBP-Cas9

    Plasmid

    #162726

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 C-FKBP-Cas9

    Plasmid

    #162729

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

    Plasmid

    #176123

    Purpose

    Constitutively active, mitochondrial localized RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G14V

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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