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KTK_009

Plasmid

#180518

Purpose

Empty backbone for D1.1 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_137

Plasmid

#180521

Purpose

Empty backbone for D1.2 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_010

Plasmid

#180519

Purpose

Empty backbone for D1.2 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_048

Plasmid

#180564

Purpose

Entry vector containing L3S1P00

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00_E1.4b

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_033

Plasmid

#180545

Purpose

Entry vector containing LuxR

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

LuxR

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_016

Plasmid

#180523

Purpose

Empty backbone for D2.2b cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_015

Plasmid

#180522

Purpose

Empty backbone for D2.2a cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EBFP_Nick_Dual_sgRNA

Plasmid

#178094

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EBFP nick sgRNAs

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EGFP_Nick_Dual_sgRNA

Plasmid

#178095

Purpose

Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EGFP nick sgRNAs

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Tpi1

Plasmid

#175940

Purpose

knockout mouse Tpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Tpi1 gRNA (Tpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-Olfr2

Plasmid

#175935

Purpose

knockout mouse Olfr2

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Olfr2 gRNA (Or6a2 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RhoJQ79L-deltaCaax

Plasmid

#176110

Purpose

Constitutively active, nucelar localized RhoJ

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RhoJ (RHOJ Human)

Expression

Mammalian

Mutation

Q79L

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-hPMEL

Plasmid

#174607

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the human hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

expresses palmitoylated GFP w/C-term fusion of the LLO190 epitope and human hPMEL epitope

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#1

Plasmid

#170834

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#2

Plasmid

#170835

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Eomes gRNA#3

Plasmid

#170836

Purpose

Cas9-mediated knockout of Eomes in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Eomes (Eomes Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Six1

Plasmid

#158532

Purpose

Expresses mouse Six1 in mammalian cells

Depositor

Neil Segil

Article

Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

Insert

SIX Homeobox 1 (Six1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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YF

Plasmid

#171459

Purpose

Expresses YF, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVpic2hygro-EGFP-FF2

Plasmid

#164921

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_V577D-talin1 (1-2541)

Plasmid

#166115

Purpose

expresses talin-1-V577D in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

V577D-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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