165,075 results

1. GPI-RpHLuorin2

   Plasmid

   #171721

   Purpose

   GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to GPI anchor
   

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdest EGFP Neo

   Plasmid

   #223052

   Purpose

   EGFP gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUB_smFLAG_KDM5B_MS2

   Plasmid

   #81084

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_KDM5B_24xMS2
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-HA-Nrxn1beta AS4(+)

   Plasmid

   #59410

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(+) (Nrxn1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-IPP1-His

   Plasmid

   #124137

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Inorganic pyrophosphatase (IPP1 Budding Yeast)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. HMA-APOBEC1-Nb

   Plasmid

   #226012

   Purpose

   For expression of His-MBP-APOBEC1-Nanobody protein in E.coli

   Depositor

   Eugene Yeo

   Article

   Liang et al Nat Commun. 2024 Aug 16;15(1):7067. doi:

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Tags

   His-MBP and antiRabbit-nanobody

   Expression

   Bacterial

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #219782

   Purpose

   CaST-IRES for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIZ-Flag6His-BmAgo3

   Plasmid

   #50559

   Purpose

   Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

   Depositor

   Yukihide Tomari

   Article

   Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

   Insert

   BmAgo3
   

   Tags

   6xHIS and Flag

   Expression

   Insect

   Promoter

   OpIE2

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMHE-miRFP-MAP4-MTBD

    Plasmid

    #171487

    Purpose

    T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    miRFP

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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