We narrowed to 8,607 results for: Ott;

1. LA601: pMVP (L3-L2) P2A-Puro + WPRE

   Plasmid

   #121785

   Purpose

   pMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectors

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-Puro + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

   Plasmid

   #47452

   Purpose

   AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE BB(N136_0.5NI_C63)-SID4X
   

   Use

   AAV; Tale

   Tags

   2A_phiLOV2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47446

   Purpose

   AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE BB(N136_0.5HD_C63)-VP64
   

   Use

   AAV; Tale

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47445

   Purpose

   AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE BB(N136_0.5NN_C63)-VP64
   

   Use

   AAV; Tale

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. KZ501: pMVP (L3-L2) P2A-Puro + polyA

   Plasmid

   #121780

   Purpose

   pMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-Puro + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCHR-CD5Flag2-P2A-smAID-mClover

   Plasmid

   #110657

   Purpose

   Lentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degron

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CD5Flag2-P2A-AID-mClover
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. KY901: pMVP (L3-L2) P2A-mCherry + polyA

   Plasmid

   #121766

   Purpose

   pMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   P2A-mCherry + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #48253

   Purpose

   LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   CRY2PHR-NLS-VP64
   

   Use

   AAV; Tale

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINK-OTUD2 (OTU, aa 132-314)

   Plasmid

   #61410

   Purpose

   Expresses human OTUD2 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-131 and aa 315-348.

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2131

    Plasmid

    #111895

    Purpose

    ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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