We narrowed to 31,286 results for: son
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Plasmid#204215PurposePlasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.DepositorInsertCas13bt3 and U6.SapI sgRNA cloning site
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationCodon optimisation by GenScript toolPromoterCMV/U6Available SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK62
Plasmid#228122PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-YE1-BE4max
Plasmid#198549PurposeExpresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NANOS3-T2A-mVenus-PuroTK
Plasmid#222903PurposeHomology directed repair template for knocking in mVenus reporter to NANOS3.DepositorInsertmVenus
UseCRISPRAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TCF4 in pENTR
Plasmid#216569Purposegateway cloning donor vector containing TCF4DepositorInsertTCF4 (TCF4 Human)
UseGateway CloningAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-oROS-HT-CaaX
Plasmid#216417PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.DepositorInsertoROS-HT-CaaX
ExpressionMammalianPromoterCMVAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-T2A-GFP
Plasmid#193781PurposeTricistronic expression of dCas9-EGFP-BSDDepositorInsertdead Cas9
UseLentiviralTagsFLAGExpressionMammalianPromoterIn frame with existing Cas9 promoterAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA
Plasmid#211817PurposeAAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNADepositorInsertN-terminus of SpCas9-ABE8e and tracrRNA
UseAAVExpressionMammalianPromoterchicken β-actin promoter and U6 promoterAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR023_PIE-PUM2
Plasmid#204819PurposeExpression PIE-PUM2 fusion protein in mammalian cellsDepositorInsertPumilio 2 (PUM2 Human)
TagsHA-Flag-rApobec1 and huADAR2ddExpressionMammalianPromoterchicken β-actin promoterAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb37
Plasmid#205515PurposeNuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertnuc-Nb37
TagsNuclear localization signal (NLS) and mCherryExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre
Plasmid#203839PurposeFlp-dependent enzymatically inactive Cre and mScarlet-KASH expressionDepositorInsertdCre
UseAAVTagsmScarlet-KASH-2AMutationEnzymatically inactive CreAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-H2B-tdTomato-SRT
Plasmid#203393PurposeAAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cardsDepositorInsertH2B fused piggyBac tdTomato self-reporting transposon
UseAAVTags3X SV40 and H2BExpressionMammalianPromoterEf1aAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-FKBP-PIP5K1C_Kina-HD
Plasmid#202741Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:FKBP1A(3-108):[GGSA]4GG:PIP5K1C(79-366)(D101R/R304D) (PIP5K1C Human)
ExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)
Plasmid#197893PurposeCan be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertmCherry
UseAAVMutationN/APromoterEF1aAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD5
Plasmid#195843PurposeExpresses human FZD5 with an N-terminal HiBiTDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD3
Plasmid#195841PurposeExpresses human FZD3 with an N-terminal HiBiT; contains I292V and V495IDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)
Plasmid#189791PurposeExpress wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-NCreIntN-T2A-mTagBFP2
Plasmid#160500PurposeExpresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CoV2-Omicron BA.1 spike
Plasmid#194602PurposeMammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)DepositorInsertCoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only