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pEF1-H2B-mRFP1-pcdk1-EGFP1-FRT

Plasmid

#247334

Purpose

Expresses histone H2B fused with mRFP1, together with monomeric EGFP

Depositor

Kazuhiro Maeshima

Article

Hihara et al Cell Rep. 2012 Dec 27;2(6):1645-56. doi:

Insert

H2B (H2BC11) (H2BC11 Synthetic, Human)

Tags

mRFP1

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PB-EF1-H4-HaloTag-IRES-Neo

Plasmid

#247450

Purpose

Expresses wild-type H4-Halo under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(Q5,8,12,16)-HaloTag-FRT

Plasmid

#247454

Purpose

Expresses wild-type H4(Q5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(Q5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4Δ1-19-HaloTag-FRT

Plasmid

#247456

Purpose

Expresses wild-type H4(Δ1-19)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4Δ1-19 lacks the initial 19 residues at the N-te…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-PA-GFP-H4-FRT

Plasmid

#247333

Purpose

Expresses histone H4 fused with photoactivatable-GFP

Depositor

Kazuhiro Maeshima

Article

Hihara et al Cell Rep. 2012 Dec 27;2(6):1645-56. doi:

Insert

H4 (H4C3) (H4C3 Human)

Tags

PA-GFP

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF5-H4(R5,8,12,16)-HaloTag-FRT

Plasmid

#247453

Purpose

Expresses wild-type H4(R5,8,12,16)-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Semeigazin et al Histochem Cell Biol. 2024 Jul;162(1-2):2

Insert

H4 (H4C1) (H4C1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

H4(R5,8,12,16) has four mutations in the N-termin…

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-SP140L-Brd 487

Plasmid

#219385

Purpose

Transcription factor SP140L with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

SP140L (SP140L Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-sgRXRa4-1; EF1a-mScarlet

Plasmid

#242889

Purpose

For lentiviral Crispr/Cas9 mediated knockout of mouse RXRa, using a guide RNA against exon 4, coupled to EF1a-driven mScarlet

Depositor

Elaine Fuchs

Article

Stewart et al Nature. 2024 Sep;633(8029):407-416. doi:

Insert

sgRNA targeting Rxra

Use

CRISPR and Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-ITGB1(YYFF)-mRuby2

Plasmid

#215447

Purpose

Gateway entry vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Tags

mRuby2

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-ITGB1(YYFF)-mRuby2

Plasmid

#215449

Purpose

Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

CAG

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

Plasmid

#235307

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#235303

Purpose

Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#235304

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

Plasmid

#235308

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

Plasmid

#235305

Purpose

Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

Plasmid

#235306

Purpose

Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET14-H2Kb-ST3

Plasmid

#223221

Purpose

Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)

Tags

Spytag

Expression

Bacterial

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-Cherry in pmCherryN1

Plasmid

#187291

Purpose

Express pmCherry-IL2-AH-deltaC2

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-PROTOP

Plasmid

#217091

Purpose

Donor plasmid of PROTOP Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of PROTOP transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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