We narrowed to 15,978 results for: grna

1. pHTganA-Cpf1

   Plasmid

   #158647

   Purpose

   Constitutive transcription of FnCpf1 and ganA crRNA

   Depositor

   Wenjing Cui

   Insert

   Cpf1, ganA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Expression

   Bacterial

   Promoter

   P43

   Available Since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pADsacA

   Plasmid

   #158649

   Purpose

   Constitutive transcription of sacA crRNA and GFP

   Depositor

   Wenjing Cui

   Insert

   sacA crRNA
   

   Use

   CRISPR and Synthetic Biology; Shuttle vector baci…

   Expression

   Bacterial

   Promoter

   Pveg

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT2-3X-FLAG-H3.1-K27M-BFP

   Plasmid

   #209439

   Purpose

   SB-transposable plasmid expressing a FLAGGED mutant histone H3.1-K27M with BFP

   Depositor

   Maria Castro

   Insert

   H3.1-K27M
   

   Expression

   Mammalian

   Mutation

   K27M

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. tet-pLKO.puro_shLuc

   Plasmid

   #110422

   Purpose

   shLuc control, silence Luc gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   Luciferase
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMKO.1 puro PTEN shRNA

   Plasmid

   #10669

   Depositor

   William Hahn

   Insert

   PTEN shRNA (PTEN Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1-blast shGFP

   Plasmid

   #110419

   Purpose

   shGFP control, silence GFP gene, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GFP
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 (RNA PolIII)

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-GFP-miRE_shRen-Puro

   Plasmid

   #135683

   Purpose

   Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-H3.3-G34R-FLAG-BFP

   Plasmid

   #209440

   Purpose

   Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34R with BFP

   Depositor

   Maria Castro

   Insert

   H3.3-G34R
   

   Expression

   Mammalian

   Mutation

   G34R

   Available Since

   Nov. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-GFP-miRE_shSCR-Puro

   Plasmid

   #135689

   Purpose

   Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection

   Depositor

   Florian Karreth

   Insert

   shScrambled
   

   Use

   Lentiviral

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-tdTomato-miRE_shRen-Puro

    Plasmid

    #135687

    Purpose

    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid

    #92306

    Purpose

    Expression of humanized BsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid

    #92304

    Purpose

    Expression of humanized Mb2Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb2Cpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid

    #92305

    Purpose

    Expression of humanized Mb3Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid

    #92303

    Purpose

    Expression of humanized TsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_016

    Plasmid

    #107143

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-EF1a-GFP-miRE_shRen-Blast

    Plasmid

    #135682

    Purpose

    Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6-BbsI-crRNA

    Plasmid

    #51026

    Purpose

    Generates crRNA for use in combination with tracrRNA

    Depositor

    Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

    Insert

    U6-BbsI-crRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6-2

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-puro-hPPM1B

    Plasmid

    #185383

    Purpose

    For mammalian expression of shRNA: GAGCAGAAGAGGATGAATTTA that targets human PPM1B

    Depositor

    Ayaz Najafov

    Insert

    PPM1B (PPM1B Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HCP5

    Plasmid

    #166107

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. cTRE-GFP-miR30_shPten.1523

    Plasmid

    #135667

    Purpose

    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    shPten
    

    Use

    Mouse Targeting

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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