We narrowed to 3,034 results for: multiplex

1. pmRuby2-CrowdingLinker-Clover

   Plasmid

   #171058

   Purpose

   Expresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.

   Depositor

   Ke Xu

   Article

   Chen et al Light Sci Appl. 2021 May 8;10(1):97. doi

   Insert

   Crowdedness-sensitive linker domain
   

   Use

   Tags

   mClover2 and mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBbi-TIR1-tTA/Pur

   Plasmid

   #171683

   Purpose

   constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

   Depositor

   Randy Poon

   Article

   Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

   Inserts

   tetracycline-controlled transactivator
   Puromycin
   EF-1a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a promoter and RPBSA promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Alpha1

   Plasmid

   #118044

   Purpose

   GoldenBraid2.0 Alpha 1 destination vector

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology; Assembly of gb2.0 transcriptio…

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTubulin-miRFP670nano

   Plasmid

   #127429

   Purpose

   Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-human alpha-tubulin
   

   Use

   Tags

   miRFP670nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCCl-MCS_miniCMV_TagBFP

   Plasmid

   #134986

   Purpose

   The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

   Depositor

   Claudio Mussolino

   Article

   Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

   Insert

   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_mCherry_2A_HA_2xMS2-VP64

   Plasmid

   #68419

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   CMV

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pELuc

   Plasmid

   #118056

   Purpose

   GBPart - ELuc Luciferase CDS

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   ELuc
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRU206

   Plasmid

   #167693

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::asCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRU322

   Plasmid

   #167683

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::asCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-sgNT3/Cre

    Plasmid

    #89654

    Purpose

    Expresses a non-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    non-targeting gRNA
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. apt-CBE-3'end

    Plasmid

    #219939

    Purpose

    Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    MCP_32-aa_evo-rA1_2xUGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWS5397-CRISPRai-NatR

    Plasmid

    #182836

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Omega 1

    Plasmid

    #118046

    Purpose

    GoldenBraid2.0 Omega 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-luxGold

    Plasmid

    #229948

    Purpose

    Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxGold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMiniP

    Plasmid

    #118049

    Purpose

    GBPart - Minimal Promoter for expression in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    minimal TATA-box promoter with low basal activity
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEE392

    Plasmid

    #149282

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_mFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

18. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWS5396-CRISPRai-KanR

    Plasmid

    #182835

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRU53

    Plasmid

    #167679

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge