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pJW1787

Plasmid

#154316

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

linker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system

Use

CRISPR

Expression

Worm

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1348

Plasmid

#154311

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker for NT slot

Use

CRISPR

Expression

Worm

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1822

Plasmid

#154322

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1594

Plasmid

#121061

Purpose

TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TagRFP-T^SEC^BioTag::AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

Expression

Worm

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH51

Plasmid

#139045

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Expression

Worm

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSM04

Plasmid

#139046

Purpose

MosSCI SapTrap assembly: Cassette 3A for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (no STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSM05

Plasmid

#139050

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mkate2 (includes STOP codon)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH66

Plasmid

#139053

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

unc-54 3'UTR (unc-54 Nematode)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBSc + Shal

Plasmid

#16142

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (Y73B6BL.19 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pISlin41m(s) (lin-41 microRNA mut reporter)

Plasmid

#11907

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence mut (lin-41 Nematode)

Use

Luciferase

Expression

Mammalian

Mutation

point mutations in miRNA complementary site

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pJH2933

Plasmid

#132363

Purpose

Prgef-1 INS-3::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-3::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-3 (ins-3 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Dec. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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AP1297‐1

Plasmid

#87247

Purpose

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Depositor

Geraldine Seydoux

Article

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Tags

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ1259

Plasmid

#44479

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, [4-3] Gateway

Use

Gateway Cloning

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pOX-twk43

Plasmid

#16128

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (F32H5.2, twk-43 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pJJR50

Plasmid

#75026

Purpose

U6 promoter driven flipped + extended sgRNA expression vector

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

guide RNA, flipped and extended version

Use

CRISPR

Promoter

R07E5.16 (U6)

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ1200

Plasmid

#44485

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, MCS

Use

Multiple cloning site vector

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCM5.60

Plasmid

#19374

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fog-1 3'utr in pDONRP2RP3

Expression

Worm

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pCM3.06

Plasmid

#19602

Depositor

Geraldine Seydoux

Article

Lee et al PLoS Genet. 2007 Dec 28. 3(12):e233.

Insert

fbf-1 ORF+3' in pDONR201 (fbf-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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U371-GFP

Plasmid

#24476

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

Contains only the kinesin's motor head fused…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only