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RCAS(A) Chfz-1 WT (CT#534)

Plasmid

#13931

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

Fz 1 (FZD1 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_PV1_VP1

Plasmid

#202071

Purpose

Bacterial expression of VP1 protein from poliovirus 1 (PV1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

PV1 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pRS316-SNU114

Plasmid

#111308

Purpose

pRS316-SNU114

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Expression

Yeast

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Felicitas Proels, Martin Scaal

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Promoter

CAG

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
PLEXIN A3

Plasmid

#58238

Purpose

Riboprobe for in vitro transcription of Plexin A3

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A3 (Plxna3 Mouse)

Expression

Bacterial

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pIG-747_CD19-28z_CAR_TFAP4

Plasmid

#207489

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERP2-hcGAS(wt)

Plasmid

#208396

Purpose

To generate stable cell line expressing human cGAS(wt) using PiggyBac transposon system

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human cGAS (CGAS Human)

Use

Piggybac transposon

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Cherry-Plekhh1

Plasmid

#187372

Purpose

Expresses human Plekhh1 labelled with Cherry

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pmx-MiGT

Plasmid

#172397

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i, Gata4, Tbx5 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-kLANA-puro

Plasmid

#131699

Purpose

expresses the KHSV LANA promoter driving puromycin resistance

Depositor

Britt Glaunsinger

Article

Hartenian et al bioRxiv 585984

Insert

LANA promoter

Expression

Mammalian

Promoter

LANA

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-Emi1_control UTRs

Plasmid

#67925

Purpose

Expresses mCherry-Emi1 with control UTRs in mammalian cells

Depositor

Ron Vale

Article

Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

Insert

mCherry-Emi1 (FBXO5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pIhh1.3K-Luc

Plasmid

#53604

Purpose

Reporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

Ihh promoter

Use

Luciferase; Reporter

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only
35 pCR vegf 121

Plasmid

#13060

Depositor

Nathan Lawson

Article

Lawson et al Dev Cell. 2002 Jul . 3(1):127-36.

Insert

vegf121

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-miR30-shJAG1 #1

Plasmid

#171196

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #1 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #1

Use

Retroviral

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pH7m34GW-35S:SNACS

Plasmid

#203576

Purpose

Plant SnRK2 kinase activity FRET sensor in plant expression vector

Depositor

Julian Schroeder

Article

Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

Insert

SNACS

Tags

StrepII

Expression

Plant

Mutation

none

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pZJQIBEBT003

Plasmid

#140053

Purpose

The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

Depositor

Jia Zhang

Article

Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

Insert

eMutS-L157C-G233C-CBM-EGFP

Tags

His-Tag

Expression

Bacterial

Mutation

L157C, G233C

Promoter

T7

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry

Plasmid

#135598

Purpose

Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-Lifeact-GFP

Plasmid

#135601

Purpose

Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Promoter

Unknown

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-loxP-GRAB_DA2m-loxP

Plasmid

#189751

Purpose

Excludes sensor expression in Cre-positive neurons

Depositor

Yulong Li

Article

Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

Insert

Dopamine sensor GRAB_DA2m

Use

AAV

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mCerulean

Plasmid

#185807

Purpose

Mitochondrial targetted (COX8) mCerulean

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mCerulean

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-mVenus

Plasmid

#185808

Purpose

Mitochondrial targetted (COX8) mVenus

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-mVenus

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2-FLAG

Plasmid

#91944

Purpose

Bacterial expression of maize SAE1 and SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPS934

Plasmid

#8855

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NUF2-sGFP (NUF2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pSB-XPRESSO-CaViar

Plasmid

#237299

Purpose

Sleeping Beauty XPRESSO vector for CaViar expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

CaViar

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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