We narrowed to 4,318 results for: Pet2;

1. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-T7-6h-GST-α-Synuclein

   Plasmid

   #225224

   Purpose

   Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   GST
   α-Synuclein
   

   Tags

   6xHis Tag and TEV Cleavage Site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Tags

   C-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET263-pUC57 24xMS2V7

   Plasmid

   #140705

   Purpose

   MS2V7 cloning

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   24xMS2V7
   

   Use

   Cloning vector

   Mutation

   24 MS2 loops V7, high-affinity C-variant, inter-s…

   Promoter

   None

   Available Since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21-HIS-Tol2

   Plasmid

   #112486

   Purpose

   functional recombinant Tol2 protein from E.coli

   Depositor

   Stephen Ekker

   Article

   Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

   Insert

   Tol2 Transposase (tpase Synthetic)
   

   Tags

   HIS/6x

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28c-F30-Broccoli

   Plasmid

   #66788

   Purpose

   Expresses Broccoli in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Broccoli in the F30 scaffold
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-2H12.D11

   Plasmid

   #221145

   Purpose

   His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MinE-His

   Plasmid

   #133623

   Purpose

   Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

   Insert

   MinE-His (minE E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Cas9-His

    Plasmid

    #98158

    Purpose

    Purification of Cas9 protein

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

    Insert

    NLS-Cas9-NLS
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a hMDH1v3 (+TEV)

    Plasmid

    #204229

    Purpose

    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH1v3 (MDH1 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Inserted a G after ATG to maintain kozak site

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet26-MBP-ELIC

    Plasmid

    #39239

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    Eriwinia chrysanthemi - pentameric ligand gated ion channel
    

    Tags

    His-10 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23a-H6-TEV-cAbl

    Plasmid

    #214234

    Purpose

    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    ABL1 Kinase domain (ABL1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-mTurquoise2-CNA35

    Plasmid

    #61602

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mTurqouise and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Jin Wang, Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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