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pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE
Plasmid#111387PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)DepositorInsertsUseAAVTagsHA and MycExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF25_IRES-mCherry
Plasmid#198386PurposeRNF25 expressionDepositorInsertRNF25 (RNF25 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR V5-RNF14_IRES-mCherry
Plasmid#198384PurposeRNF14 expressionDepositorInsertRNF14 (RNF14 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK305
Plasmid#66812PurposeIVT vector for Fluc mRNADepositorInsertFirefly luciferase
UseLuciferase; Mrna ivtTagsExpressionMutationPromoterT7Available sinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTPH418b
Plasmid#185725PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
sABE v3.22_N
Plasmid#209553PurposepCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpADepositorInsertTadA-8e(2-76)-2xFRB
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTPH405c
Plasmid#185726PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C) single PAM linker
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-ZYG11B
Plasmid#110550Purposeexpresses a FLAG-tagged human ZYG11B protein in mamalian cellsDepositorInsertZYG11B (ZYG11B Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG-ZYG11A
Plasmid#110551Purposeexpresses a FLAG-tagged human ZYG11A protein in mamalian cellsDepositorInsertZYG11A (ZYG11A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF6 rglut1 flag complete
Plasmid#89571Purposerglut1 flag completeDepositorInsertrglut1 flag complete (Slc2a1 Rat)
UseTagsExo FacialExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
EC.SurA.TYB1.a172-274.wt
Plasmid#15934DepositorInsertPeptidyl prolyl isomerase domain 1 of SurA (surA E. coli)
UseTagsinteinExpressionBacterialMutationwild typePromoterAvailable sinceNov. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609-M2WA_T
Plasmid#147634PurposeInsect Expression of HsTNRC6C_1207-1609-M2WADepositorInsertHsTNRC6C_1207-1609-M2WA (TNRC6C Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1609_R
Plasmid#147410PurposeInsect Expression of HsTNRC6C_1207-1609DepositorInsertHsTNRC6C_1207-1609 (TNRC6C Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGPN
Plasmid#187482PurposeExpression vector - GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Omicron.BA.5-RBD
Plasmid#213478PurposeMammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variantDepositorInsertpCAGGS/Omicron.BA.5 RBD (S )
UseTags6 His TagExpressionMammalianMutationG339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…PromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-OMICRON
Plasmid#180593PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertSpike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE
Plasmid#111395PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)DepositorInsertsUseAAVTagsEGFP and MycExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE
Plasmid#111393PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLCa
Plasmid#196912PurposeVisualization of clathrin-coated pit formation in living mammalian cellsDepositorInsertClathrin-Coated light chain
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only
