We narrowed to 13,271 results for: cDNA

1. pCDNA5_miniTurbo-C12orf49

   Plasmid

   #155111

   Purpose

   transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-HA-mMcl1

   Plasmid

   #25714

   Depositor

   Roger Davis

   Article

   Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

   Insert

   mMCL1 (Mcl1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 HA-rnSOX9

   Plasmid

   #62972

   Purpose

   Expresses HA-tagged rat SOX9

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3+ hSlo2.2

   Plasmid

   #16214

   Depositor

   Larry Salkoff

   Insert

   KCNT1 potassium channel, subfamily (KCNT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   This construct lacks the first 53aa when compared…

   Promoter

   Available Since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Wnt4

   Plasmid

   #35910

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt4 (WNT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent Mutation C (492) --> T

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. NXPE1_pcDNA3.4-TOPO

   Plasmid

   #226841

   Purpose

   Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.

   Depositor

   Bert Vogelstein

   Insert

   NXPE1 (NXPE1 Human)
   

   Use

   Tags

   x6 HIS and an IL2 secretion signal both N termina…

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-miR17

   Plasmid

   #21113

   Depositor

   Joshua Mendell

   Article

   Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

   Insert

   miR-17 (MIR17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.2/NS4B

   Plasmid

   #120823

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS4B ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-hNoxa1

    Plasmid

    #58531

    Purpose

    expresses human Noxa1 in mammalian cells

    Depositor

    Karl-Heinz Krause, Botond Banfi

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    NADPH oxidase activator 1 (NOXA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.3_SARS2_BA.2.12.1

    Plasmid

    #186809

    Purpose

    pseudovirus plasmid that expresses spike of BA.2.12.1

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (BA.2.12.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L452Q, S704L on the basis of BA.2, c-terminal 18 …

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-D1cpV

    Plasmid

    #37479

    Depositor

    Roger Tsien, Amy Palmer

    Article

    Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

    Insert

    D1cpV second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-rxRFP

    Plasmid

    #67830

    Purpose

    Redox-Sensitive Red Fluorescent Protein

    Depositor

    Huiwang Ai

    Article

    Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

    Insert

    Redox-Sensitive Red Fluorescent Protein
    

    Use

    Tags

    His Tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-3xhfYFP

    Plasmid

    #186544

    Purpose

    Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Hyperfolder YFP (3X)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.2/NS3

    Plasmid

    #120822

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS3 ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-EphB3-Fc

    Plasmid

    #200985

    Purpose

    Mammalian expression plasmid for soluble EphB3 fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB3 (EPHB3 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Soluble ectodomain of EphB3

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TLS 2: hTLS.pCDNA1

    Plasmid

    #21828

    Depositor

    David Ron

    Article

    Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

    Insert

    TLS (FUS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. BRAC/pcDNA3

    Plasmid

    #51967

    Purpose

    mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

    Insert

    BRAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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