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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (GFP)

Plasmid

#102944

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6f.WPRE.SV40

Plasmid

#100837

Purpose

AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-MT1-MMP-eGFP

Plasmid

#89819

Purpose

Express in MT1-MMP mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

MT1-MMP (MMP14 Human)

Use

Retroviral

Tags

eGFP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-Nanoluc-ccdB

Plasmid

#87078

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Lentiviral

Tags

Nanoluc luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG PTEN

Plasmid

#78777

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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