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pBN077

Plasmid

#167958

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN078

Plasmid

#167959

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E

Promoter

araBAD

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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C238-E07: attB2r-SV40polyA-attB3

Plasmid

#162917

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SV40 polyadenylation sequence

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E24: attB2r-Strep2-attB3

Plasmid

#162913

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Strep2 epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E36: attB4-CMV51p>-attB1r

Plasmid

#162927

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

optimized CMV promoter

Use

Synthetic Biology

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E06: attB2r-FLAG-attB3

Plasmid

#162910

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

FLAG epitope tag

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-PLY-WT-no charge

Plasmid

#160539

Purpose

Expression of inserted PLY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

PLY-WT-no charge

Tags

His-Tag

Expression

Bacterial

Mutation

K279A, Q280A, D283A, N284A

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-Cdt1

Plasmid

#122342

Purpose

Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Cdt1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFD-AI(TVMV)

Plasmid

#137111

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(TVMV)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/CURT_fluoB

Plasmid

#117999

Purpose

Expression of CURT_fluoB controlled by the ARA promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

CURT_fluoB

Expression

Bacterial

Promoter

ARA

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-LAP-BUB1

Plasmid

#115903

Purpose

Used for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

BUB1 (BUB1 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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MPS-1-cmyc

Plasmid

#113059

Purpose

Expresses worm Myc-tagged MPS-1 in mammalian cells

Depositor

Federico Sesti

Article

Wang et al Biophys J. 2007 Nov 1;93(9):3083-91. doi

Insert

MiRP K+ channel accessory Subunit 1 (mps-1 Nematode)

Tags

c-Myc

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-HSE1-iGFPx6

Plasmid

#105259

Purpose

Plasmid for pop-in/pop-out replacement of Hse1 with Hse1-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Hse1 portion (HSE1 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-MUP1-iYFP

Plasmid

#105266

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-iYFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Mup1 portion (MUP1 Budding Yeast)

Tags

iYFP

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049d

Plasmid

#96862

Purpose

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT049e

Plasmid

#96863

Purpose

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (19 nt blocker with bulges, RFP activation)

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1895 HC_Kan_Kozak-ATG_p6

Plasmid

#100680

Purpose

part designed to occupy position 6 of EMMA. Functional category: Kozak sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Kozak-ATG

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pms5102(JBEI-10536)

Plasmid

#87969

Purpose

jStack yeast assembly into plant vectors

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Insert

{L_AtHsp18.2}

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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