We narrowed to 19,676 results for: Cre

1. pRS416 Gal TDP43 M337V

   Plasmid

   #27460

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Expression

   Yeast

   Mutation

   Methionine 337 mutated to Valine (M337V)

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pRS416 Gal TDP43 Q331K

   Plasmid

   #27461

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Expression

   Yeast

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Available Since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-CaMKII-nirButterfly

   Plasmid

   #136591

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded voltage indicator nirButterfly

   Depositor

   Vladislav Verkhusha

   Article

   Monakhov et al ACS Chem Neurosci. 2020 Nov 4;11(21):352

   Insert

   nirButterfly
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2puro-Tbxa2r

   Plasmid

   #170304

   Purpose

   A knock-out vector for the mouse Tbxa2r

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Tbxa2r gene.
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. RhopH3-bio

   Plasmid

   #47781

   Purpose

   Expresses enzymatically monobiotinylated full-length RhopH3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RhopH3
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

   Plasmid

   #121835

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC120

   Plasmid

   #118892

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   MBP, cleavable with 3C protease and mGFP, HIS6, c…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. DCT.VV.Orf42

   Plasmid

   #12730

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf42
   

   Expression

   Mammalian

   Available Since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pXPA-fapO-RgTALsyn

   Plasmid

   #100946

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   GAP (Constitutive)

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid

    #121824

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 782 pMEActMEK

    Plasmid

    #31166

    Depositor

    Nathan Lawson

    Article

    Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

    Insert

    MEK (MAP2K1 Human)
    

    Use

    Entry vector

    Mutation

    Ser218Glu Ser222Asp; activated MEKK

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16

    Plasmid

    #89870

    Purpose

    expresses DRD2-iTango component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-PKM2-G415R

    Plasmid

    #122693

    Purpose

    Express His-tagged PKM2 (G415R) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    G415R

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneoLuc-PP1A

    Plasmid

    #128348

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    PP1A (PPP1CA Human)
    

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sema4f.b-Fc-His

    Plasmid

    #72159

    Purpose

    Expresses the extracellular region of the Sema4F, isoform b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4f.b (Sema4f Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sema5a-AP-His

    Plasmid

    #72035

    Purpose

    Expresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema5a (Sema5a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. DCT.VV.Orf196

    Plasmid

    #12884

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf196
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

18. Sema4b-Fc-His

    Plasmid

    #72155

    Purpose

    Expresses the extracellular region of the Sema4B protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4b (Sema4b Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Nrp1-AP-His

    Plasmid

    #71971

    Purpose

    Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp1 (Nrp1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. JP705: pMVP/SB/Neo-DEST

    Plasmid

    #121866

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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