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TFORF0386

Plasmid

#142611

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA4 (SMARCA4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Collybistin [L120/12R]

Plasmid

#177575

Purpose

Mammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GAD67 [L127/8R]

Plasmid

#177475

Purpose

Mammalian Expression Plasmid of anti-GAD67 (Human). Derived from hybridoma L127/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GAD67 (Homo sapiens) recombinant mouse monoclonal antibody (GAD1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Ankyrin-G scFv [N106/20]

Plasmid

#190525

Purpose

Mammalian Expression of Ankyrin-G scFV. Derived from hybridoma N106/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-G (Homo sapiens) recombinant scFV (ANK3 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Ankyrin-B scFv [N105/17]

Plasmid

#190524

Purpose

Mammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Ankyrin-B scFv [N105/13]

Plasmid

#190523

Purpose

Mammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Ankyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-G [N106/36]

Plasmid

#190297

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (Human). Derived from hybridoma N106/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-G (Homo sapiens) recombinant Mouse monoclonal antibody (ANK3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Neurexin-1-Beta scFv [N170A/1]

Plasmid

#190534

Purpose

Mammalian Expression of Neurexin-1-Beta scFV. Derived from hybridoma N170A/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neurexin-1-Beta (Homo sapiens) recombinant scFV (NRXN1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/43]

Plasmid

#190489

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/38]

Plasmid

#190487

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Homer1L/S scFv [L113/130]

Plasmid

#190501

Purpose

Mammalian Expression of Homer1L/S scFV. Derived from hybridoma L113/130.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L/S (Mus musculus) recombinant scFV (Homer1 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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GABA(A)R, gamma2 scFv [N452/81]

Plasmid

#182088

Purpose

Mammalian Expression of GABA(A)R, gamma2 scFv. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GABA(A)R, gamma2 (Homo sapiens) (GABRG2 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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CASPR/Neurexin IV scFv [K65/35]

Plasmid

#182068

Purpose

Mammalian Expression of CASPR/Neurexin IV scFv. Derived from hybridoma K65/35.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting CASPR/Neurexin IV (Rattus norvegicus) (Cntnap1 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-nNOS/NOS1 [L121/25R]

Plasmid

#177466

Purpose

Mammalian Expression Plasmid of anti-nNOS/NOS1 (Human). Derived from hybridoma L121/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-nNOS/NOS1 (Homo sapiens) recombinant mouse monoclonal antibody (NOS1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-PHB-6

Plasmid

#122232

Purpose

Expresses shRNA targeting the coding sequence of human PHB

Depositor

Alex Kentsis

Article

Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

Insert

shRNA targeting PHB (see partial depositor seq) (PHB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SAP102 [N19/2R]

Plasmid

#177510

Purpose

Mammalian Expression Plasmid of anti-SAP102 (Rat). Derived from hybridoma N19/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SAP102 (Rattus norvegicus) recombinant mouse monoclonal antibody (Dlg3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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IRSp53/BAIAP2 scFv [L117/1]

Plasmid

#182075

Purpose

Mammalian Expression of IRSp53/BAIAP2 scFv. Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting IRSp53/BAIAP2 (Homo sapiens) (BAIAP2 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Collybistin [L120/30R]

Plasmid

#177465

Purpose

Mammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Brevican [N294A/10R]

Plasmid

#177528

Purpose

Mammalian Expression Plasmid of anti-Brevican (Rat). Derived from hybridoma N294A/10R.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Brevican (Rattus norvegicus) recombinant mouse monoclonal antibody (Bcan Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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