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pCMV5 Flag p38 alpha

Plasmid

#20786

Depositor

Roger Davis

Publication

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV5 Flag p38 alpha (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1 ASA

Plasmid

#13479

Depositor

Anton Bennett

Publication

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MKP-1ASA (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Arginine 53 to Alanine, Changed Arginine…

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-p15ABAD- YFP

Plasmid

#186571

Purpose

p15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Publication

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

BAD promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CLP1

Plasmid

#155462

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLP1 (CLP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-mNeonGreen

Plasmid

#105713

Purpose

AAV packaging vector to express mNeonGreen in astrocytes.

Depositor

Pantelis Tsoulfas

Insert

mNeonGreen

Use

AAV

Promoter

Minimal GFAP promoter

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-hIFITM3-Y20A

Plasmid

#58462

Purpose

Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Publication

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pEBB-BT

Plasmid

#36295

Depositor

Ezra Burstein

Publication

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Type

Empty backbone

Tags

BT-tag (peptide sequence from P.freudenreichii tr…

Expression

Mammalian

Promoter

EF1alpha

Available since

June 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lentiviral FLAG-CaMKKbeta

Plasmid

#32458

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

FLAG

Available since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-1: shALAS-1

Plasmid

#22750

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

shALAS-1 (Alas1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only
pMV1 pEF1a-HGF

Plasmid

#188748

Purpose

Vector constitutively expressing HGF

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

HGF (HGF Human)

Expression

Mammalian

Promoter

pEF1a

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Publication

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCS within donor template

Use

CRISPR

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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SF3A2

Plasmid

#156001

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SF3A2 (SF3A2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DHX35

Plasmid

#155538

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX35 (DHX35 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CNOT3

Plasmid

#155467

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CNOT3 (CNOT3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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DCP1A

Plasmid

#155498

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DCP1A (DCP1A Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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SMG6

Plasmid

#156020

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SMG6 (SMG6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Publication

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
Vh-HA-DHFR

Plasmid

#20217

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHFR (folA E. coli)

Tags

HA and Vh

Expression

Mammalian

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Neurl1Rm-Flag pcDNA3.1

Plasmid

#17319

Depositor

Joan Conaway, Ronald Conaway

Publication

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article