We narrowed to 88,257 results for: MAL

1. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_OHu19986C_myc tag

   Plasmid

   #183174

   Purpose

   used to make AAV vectors for hsTRPA1 tagged with myc

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Insert

   hsTRPA1 (TRPA1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MC4R

   Plasmid

   #233630

   Purpose

   encodes the human melanocortin 4 receptor, without any tags

   Depositor

   Christian Gruber

   Article

   Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

   Insert

   MC4R (MC4R Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/T7

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2333_Cas9-CtIP-dnRNF168

   Plasmid

   #200254

   Purpose

   This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

   Depositor

   Claudio Mussolino

   Article

   Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

   Insert

   Cas9-CtIP-dnRNF168
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

   Plasmid

   #205631

   Purpose

   Expression of TnpBmax in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB optimized for mammalian expression
   

   Use

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. A2_uSFFV

   Plasmid

   #153415

   Purpose

   Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

   Depositor

   Maki Nakayama

   Article

   Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

   Insert

   A*02:01-P2A-human beta2 microglobulin (B2M Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/Flag-METTL3

   Plasmid

   #53739

   Purpose

   For expression of METTL3 (methyltransferase-like 3) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK322 (TOP2A-mAC (Hygro))

   Plasmid

   #140653

   Purpose

   TOP2A tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. nLuc_AP4_TEVs_P3mS

    Plasmid

    #119299

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    

    Use

    Luciferase

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AP4mS_PPVs_P3_cLuc

    Plasmid

    #119300

    Purpose

    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MAP1LC3B

    Plasmid

    #137757

    Purpose

    Expression of LC3B

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-mBATF

    Plasmid

    #34575

    Depositor

    Peter Murray

    Article

    Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

    Insert

    BATF (Batf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

15. AAV-hSyn-Soma-jGCaMP8m

    Plasmid

    #169257

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    Soma-jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-E-cadherin promoter

    Plasmid

    #61798

    Purpose

    Luciferase reporter containing the mouse E-cadherin promoter

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    -178/+92 fragment of the mouse Cdh1 promoter (Cdh1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

17. p2T-CMV-miniSdd7-BE4max-BlastR

    Plasmid

    #204851

    Purpose

    For cytosine base editing using miniSdd7 in mammalian cells

    Depositor

    Caixia Gao

    Article

    Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

    Insert

    miniSdd7-nSpCas9(D10A)-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV, SV40, EM7

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-SFFV-dCas9-BFP

    Plasmid

    #46910

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRluc-LC3G120A

    Plasmid

    #105003

    Purpose

    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Glycine 120 mutated to alanine, prevents lipidati…

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC49

    Plasmid

    #133060

    Purpose

    Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

    Depositor

    Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    Tau.K18(LM)
    

    Use

    Lentiviral

    Tags

    mScarletI

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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