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We narrowed to 15,275 results for: grn

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Showing: 9321 - 9340 of 15275 results

  4. pSCL.180

    Plasmid
    #185001
    Purpose
    Express -Eco1 AAVS1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    AAVS1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185001-plasmid-map-sequence-id-376682

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  5. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

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  6. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

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  7. peSCBE3-NG

    Plasmid
    #218163
    Purpose
    This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Publication
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218163-plasmid-map-sequence-id-433173

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  10. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Publication
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  11. B270 + SPRTN sgSTOP

    Plasmid
    #100718
    Purpose
    B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting SPRTN (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100718-plasmid-map-sequence-id-193654

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  12. B52 + TIMELESS sgSTOP

    Plasmid
    #100713
    Purpose
    B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting TIMELESS (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100713-plasmid-map-sequence-id-193647

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  13. B52 + PARP4 sgSTOP

    Plasmid
    #100711
    Purpose
    B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)
    Depositor
    Alberto Ciccia
    Publication
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgSTOP targeting PARP4 (cloned using BbsI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100711-plasmid-map-sequence-id-193646

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  14. pCRISPR Human CASP9 -2

    Plasmid
    #198420
    Purpose
    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein
    Depositor
    Hannah Rabinowich
    Publication
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CASP9 Specific gRNA (CASP9 Human)
    Use
    CRISPR
    Tags
    Cas9/Orange Fluorescent Protein Reporter
    Expression
    Mammalian
    Promoter
    U6; CMV
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198420-plasmid-map-sequence-id-390260

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  15. pCRISPR Human CASP9 -1

    Plasmid
    #198419
    Purpose
    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein
    Depositor
    Hannah Rabinowich
    Publication
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CASP9 Specific gRNA (CASP9 Human)
    Use
    CRISPR
    Tags
    Cas9/Orange Fluorescent Protein Reporter
    Expression
    Mammalian
    Promoter
    U6; CMV
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198419-plasmid-map-sequence-id-390148

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  17. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Publication
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  19. pTBL681 CHYRON4 integration construct

    Plasmid
    #126449
    Purpose
    To integrate the CHYRON4 locus at AAVS1 in human cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pU6/3xLacO-CHYRON4 hgRNA
    pCMV-puro
    Expression
    Mammalian
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    CMV and human U6/3xLacO
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126449-plasmid-map-sequence-id-246016

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Showing: 9321 - 9340 of 15275 results