We narrowed to 11,400 results for: 110

1. Intron-Tagging-pX330-Cas9-mCherry

   Plasmid

   #159742

   Purpose

   Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK TT FLAG-HA RBFOX2

   Plasmid

   #59769

   Purpose

   doxycycline-inducible expression of FLAG-HA-tagged human RBFOX2 in mammalian cells

   Depositor

   Phil Sharp

   Article

   Jangi et al Genes Dev. 2014 Mar 15;28(6):637-51. doi

   Insert

   RBFOX2 (RBFOX2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPC-NMYC-mTRF1

   Plasmid

   #64162

   Purpose

   Retroviral vector expressing mouse TRF1 with N-terminal Myc tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   mTRF1 (Terf1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pDIRECT_26H

   Plasmid

   #91150

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSV-CaMKIV

   Plasmid

   #45062

   Purpose

   Expression of CaMKIV

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   Cam Kinase IV (Camk4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   *

   Promoter

   RSV

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-IC2-N237

   Plasmid

   #51410

   Purpose

   expression of GFP-tagged N-terminal AA 1-237 of dynein intermediate chain IC2C in mammalian cells

   Depositor

   Trina Schroer

   Article

   King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

   Insert

   dynein intermediate chain IC2C (Dync1i2 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   AA 1-237

   Promoter

   CMV

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

   Plasmid

   #163881

   Purpose

   Acute Depletion of Mau2-mScarlet

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Mau2 (Mau2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_B2515b

   Plasmid

   #91073

   Purpose

   Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_B2301

   Plasmid

   #91067

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   35S

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_C2616

    Plasmid

    #91090

    Purpose

    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    At7SL

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

    Plasmid

    #166411

    Purpose

    express hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    ef-1a

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_MAD2L1_HORMA

    Plasmid

    #178480

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    MAD2L1_HORMA (MAD2L1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM33_TNKS_TANK-peptide-bind

    Plasmid

    #178489

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    TNKS_TANK-peptide-bind (TNKS Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dendra2-Vinculin-N-21

    Plasmid

    #57749

    Purpose

    Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    NM_003373.3 (VCL Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pMOD_C2517

    Plasmid

    #91086

    Purpose

    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_B2615

    Plasmid

    #91078

    Purpose

    Module B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    BsaI ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    AtU6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDDGFP2_leu2d_NRT1.1

    Plasmid

    #58332

    Purpose

    Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi:

    Insert

    NRT1.1 (NRT1.1 Mustard Weed)
    

    Use

    Tags

    GFP-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330 NIPBL gRNA

    Plasmid

    #165590

    Purpose

    Insertion of NIPBL degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    NIPBL (Nipbl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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