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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PGK CFP

Plasmid

#200429

Purpose

Mammalian expression of CFP

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

CFP

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA D463N

Plasmid

#195203

Purpose

Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

Depositor

David Bryant, Peter Parker

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Mutation

D463N

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-KRasCT

Plasmid

#175835

Purpose

Express mCherry with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*/124

Plasmid

#177807

Purpose

The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*/124

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mouse Clock delta 19

Plasmid

#177312

Purpose

expression of mutant mouse clock

Depositor

Joseph Takahashi

Article

King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

Insert

mouse clock delta 19

Expression

Mammalian

Mutation

deletion from original mClock gene (514-564aa)

Promoter

CMV

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_B6

Plasmid

#170300

Purpose

A knock-out vector for the mouse Ptgs2 in C57BL6

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-SNAPf-CCR5TC

Plasmid

#135536

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

SNAPf

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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907H1

Plasmid

#160234

Purpose

FlightN-CymR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

p-cymene repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-BE4maxCP1028-BlastR

Plasmid

#152993

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9 CP1028-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pASPIre3

Plasmid

#154844

Purpose

Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

Depositor

Markus Jeschek

Article

Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

Insert

Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator

Expression

Bacterial

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-HrasG12V

Plasmid

#89887

Purpose

Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-HRasG12V

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE5

Plasmid

#79461

Purpose

sgRNA shuttle vector; module 1

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-intein/Src

Plasmid

#71406

Purpose

Expresses Src inserted with a Npu DnaE intein (F1 construct)

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Src active kinase domain inserted with Npu DnaE intein

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-SELP0K

Plasmid

#67007

Purpose

Expresses malE-SELP0K in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-SELP0K

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b

Plasmid

#52122

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only
pET-PesaR-Plux

Plasmid

#47659

Purpose

PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-P

Use

Synthetic Biology

Expression

Bacterial

Mutation

Additional esa box downstream of the original esa…

Promoter

PesaR-P

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-AK1

Plasmid

#31290

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

adenylate kinase 1 (AK1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCY 3010-05

Plasmid

#36211

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

LEU2

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-LEU2

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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