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Showing: 1381 - 1410 of 1461 results

  5. AAVS1-iPE-Plus

    Plasmid
    #214062
    Purpose
    inducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locus
    Depositor
    Ting Zhou
    Article
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    PE-Plus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214062-plasmid-map-sequence-id-429371

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  15. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Article
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    109399-plasmid-map-sequence-id-211963

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  17. pMX-53BP1(1-1710) 3LA

    Plasmid
    #185698
    Purpose
    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    L175A, L517A, and L693A
    Promoter
    SV40
    Available since
    July 7, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185698-plasmid-map-sequence-id-365946

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  18. pMX-53BP1(1-1710) 3LA7A

    Plasmid
    #185707
    Purpose
    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.
    Depositor
    Daniel Durocher
    Article
    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.
    Insert
    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    Use
    Retroviral
    Tags
    Flag tag, HA tag, and tetracysteine tag
    Expression
    Mammalian
    Mutation
    T302A, S452A, S523A, S543A, S625A, S784A, and S89…
    Promoter
    SV40
    Available since
    July 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185707-plasmid-map-sequence-id-365947

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  20. mEmerald-Nup35-C-18

    Plasmid
    #54207
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup35 (NUP35 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. mEmerald-Nup35-N-14

    Plasmid
    #54208
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup35 (NUP35 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. WARS_pNIC-CTB10H

    Plasmid
    #153058
    Purpose
    Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    WARS (WARS1 Human)
    Use
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153058-plasmid-map-sequence-id-304478

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  23. RRM2BA

    Plasmid
    #42408
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RRM2BA (RRM2B Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  24. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Archa Fox
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97087-plasmid-map-sequence-id-189642

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  25. 4QT6

    Plasmid
    #64651
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HERC1:YTC014-A04:C231700 (HERC1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. YTC013-A06

    Plasmid
    #58756
    Purpose
    bacterial expression of PDB 4O2W
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4O2W (HERC1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1381 - 1410 of 1461 results