We narrowed to 5,262 results for: 290

1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-7

   Plasmid

   #129047

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA7 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9

   Plasmid

   #129049

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA9 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10

   Plasmid

   #129050

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA10 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

   Plasmid

   #129051

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA11 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12

   Plasmid

   #129052

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA12 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

   Plasmid

   #129043

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA3 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

   Plasmid

   #129044

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA4 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5

   Plasmid

   #129045

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA5 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6

   Plasmid

   #129046

   Purpose

   inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA6 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-CMV GFP-LC3B Q116P G120

    Plasmid

    #129290

    Purpose

    Expresses GFP-tagged deconjugation-resistant LC3B in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_ARAF_p.S214C_p.D429A

    Plasmid

    #82904

    Purpose

    Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ARAF (ARAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S214C, D429A

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223_ARAF_p.S214C

    Plasmid

    #82907

    Purpose

    Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ARAF (ARAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S214C

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_ARAF_p.D429A

    Plasmid

    #82909

    Purpose

    Gateway Donor vector containing ARAF, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ARAF (ARAF Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D429A

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid

    #208290

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpCas9(D10…

    Promoter

    CMV and T7

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_ERBB2_p.D845A

    Plasmid

    #82905

    Purpose

    Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    D845A

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-Kv1.2 pS440/pS441 phosphospecific [L64/32]

    Plasmid

    #190290

    Purpose

    Mammalian Expression Plasmid of anti-Kv1.2 pS440/pS441 phosphospecific (Human). Derived from hybridoma L64/32.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Kv1.2 pS440/pS441 phosphospecific (Homo sapiens) recombinant Mouse monoclonal antibody (KCNA2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.23 C2_14

    Plasmid

    #60290

    Purpose

    This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    EDARADD inactive enhancer (EDARADD Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCXLE-SOX2-17-p2a-KLF4

    Plasmid

    #193290

    Purpose

    episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

    Plasmid

    #172907

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Voltron2-ST
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

    Plasmid

    #172908

    Purpose

    AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

    Depositor

    GENIE Project

    Article

    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

    Has Service

    AAV1

    Insert

    Ace2N-4AA-mNeon-ST A122D
    

    Use

    AAV and Cre/Lox

    Tags

    soma localization targeting sequence

    Expression

    Mammalian

    Mutation

    A122D

    Promoter

    hSynapsin1

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge