We narrowed to 7,122 results for: ef1a

1. pLV-Hygro-EF1A-EGFP-PARP1

   Plasmid

   #176146

   Purpose

   EGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   Poly(ADP-Ribose) Polymerase 1 (PARP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1a-PTS1-IRES-Puromycin

   Plasmid

   #134665

   Purpose

   Expresses EGFP-tagged peroxisome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   tripeptide SKL (Peroxisome targeting signal 1)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVH-EF1a-GFP-P2A-SOX2

   Plasmid

   #130693

   Purpose

   Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

   Plasmid

   #193752

   Purpose

   human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

   Plasmid

   #155307

   Purpose

   Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Eef1a-1955/-1>nls::Cas9::nls

   Plasmid

   #59987

   Purpose

   Eef1a (EF1alpha) promoter driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Eef1a (EF1alpha) -1955/-1

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #125692

   Purpose

   Global labeling of inhibitory synapses (green fluorescence)

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC374 - pAAV EF1a DIO iRFP

   Plasmid

   #47626

   Purpose

   An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Infrared fluorescent protein
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

   Plasmid

   #189807

   Purpose

   Retroviral delivery of Cas9

   Depositor

   Ellen Rothenberg

   Article

   Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-N2c_envA-IRES-Neo

    Plasmid

    #172368

    Purpose

    Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    envA + Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DIO-EGFP-WPRE-pA

    Plasmid

    #37084

    Purpose

    Cre-dependent expression of EGFP

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox; Cre-on

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-fDIO-mRuby3-2A-Cre

    Plasmid

    #203844

    Purpose

    Flp-dependent Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-AAVS1-Ef1a-Puro-mScarlet

    Plasmid

    #227274

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

    Plasmid

    #171027

    Purpose

    Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1 and AAV9

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    ChrimsonR is fused to tdTomato

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-tdTomato-WPRE-pGHpA

    Plasmid

    #67527

    Purpose

    "Expresses the fluorescent protein tdTomato"

    Depositor

    Botond Roska

    Article

    Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

    Insert

    tandem dimer tomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

    Plasmid

    #201032

    Purpose

    Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-UbC-FKRP-EF1a-BSD

    Plasmid

    #205150

    Purpose

    Express UbC-driven FKRP and EF-1a driven BSD

    Depositor

    Monkol Lek

    Insert

    Fukutin-related protein (FKRP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-Con/Fon-GCaMP6F

    Plasmid

    #137122

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV5 and AAV8

    Insert

    Con/Fon-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC0045-EF1a-PguCas13b-NES-HIV

    Plasmid

    #103861

    Purpose

    Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PguCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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