We narrowed to 1,234 results for: halo

1. pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

   Plasmid

   #197943

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-G41S, D127A,L277W,F252Y

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DL147_plenti_TRE_HT-iPAK4

   Plasmid

   #187447

   Purpose

   Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   INKA-box Pak4
   

   Use

   Lentiviral

   Tags

   halo tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJB01

   Plasmid

   #111957

   Purpose

   Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Insert

   RFP
   

   Use

   Tags

   HaloTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRC02

   Plasmid

   #114681

   Purpose

   sfGFP.Strand10-Haloenzyme

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Insert

   sfGFP.Strand10-Haloenzyme
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSK573

   Plasmid

   #190881

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

   Depositor

   Gunjan Mehta, Tatiana Karpova

   Article

   Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAG.mCherry-INBox-eDHFR

   Plasmid

   #176202

   Purpose

   Single INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   mCherry-INBox-eDHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CAG.MCAK-mCherry-eDHFR

   Plasmid

   #176204

   Purpose

   MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   MCAK-mCherry-eDHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLM032

   Plasmid

   #134244

   Purpose

   Retroviral plasmid with H3.1A EGFP insert

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   Human H3.1A + eGFP/HALO retroviral tagging
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CAG.INBox-mCherry-INBox-eDHFR

   Plasmid

   #176203

   Purpose

   Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   INBox-mCherry-INBox-eDHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CAG.mCherry-INBox(AAA)-eDHFR

    Plasmid

    #176207

    Purpose

    INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    mCherry-INBoxAAA-eDHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTSK561

    Plasmid

    #190816

    Purpose

    For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

    Depositor

    Gunjan Mehta, Tatiana Karpova

    Article

    Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

    Insert

    HaloTag
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTij_sg_mmu_Med6_C_terminal

    Plasmid

    #197869

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    Med6 (Med6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTij_sg_mmu_Med1_N_terminal

    Plasmid

    #197870

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLM033

    Plasmid

    #134245

    Purpose

    Retroviral plasmid with H4 EGFP insert

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    Human H4 + eGFP/HALO retroviral tagging (HRH4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ECON

    Plasmid

    #82553

    Purpose

    Epigenetic effector construct for targeted methylation

    Depositor

    Joseph Irudayaraj

    Article

    Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    HALO and mCherry

    Expression

    Mammalian

    Mutation

    N-terminal photolyase homology region domain of C…

    Promoter

    CMV

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIPZ (M)-H2A-HT

    Plasmid

    #82517

    Purpose

    Lentivirus vector. Expresses H2A-Halotag fusion proteins in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    Histone H2A
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HT7-mTurquoise2-KDEL

    Plasmid

    #188571

    Purpose

    Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    HaloTag7
    

    Use

    Tags

    KDEL and mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid

    #20949

    Purpose

    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    halorhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIPZ (M)-HT-Cbx2

    Plasmid

    #82510

    Purpose

    Expresses Halotag-Cbx2 fusion proteins in mammalian cells

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 2 (CBX2 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAL750

    Plasmid

    #200988

    Purpose

    Inducible promoter Pxyl for Haloferax volcanii

    Depositor

    Alexandre Bisson

    Article

    Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

    Type

    Empty backbone

    Use

    Expression in haloarchaea

    Tags

    Expression

    Mutation

    Promoter

    Pfdx, Pxyl

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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