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pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Use

Tags

Antp

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(DAP10)

Plasmid

#177904

Purpose

Bacterial expression of his10-tagged human DAP10 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

DAP10 (aa 70-92 only) (HCST Human)

Use

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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3322_pETcon_SARS2_Omicron-BA2

Plasmid

#184409

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Use

Tags

6x His tag and SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 pET15b

Plasmid

#60677

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pET23a-LIR-1 D1-D2

Plasmid

#12103

Depositor

Pamela Bjorkman

Article

Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

Insert

Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acids 1−198*

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pJL1-CyPet

Plasmid

#102636

Purpose

In vitro expression of CyPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

CyPet

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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petRNA-FANCF-v3'

Plasmid

#181803

Purpose

petRNA FANCF v3'

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

ribozyme-petRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - APX

Plasmid

#89618

Purpose

Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Ascorbate dependent Tryparedoxin peroxidase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-hnRNP-F

Plasmid

#23021

Depositor

Douglas Black

Article

Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

Insert

hnRNP-F (HNRNPF Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Use

Tags

His and thrombin cleavage

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET21a-EthR

Plasmid

#51170

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

EthR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET32a-hHBEGF

Plasmid

#199234

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proheparin-binding EGF-like growth factor (HBEGF Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pET3a-hUb

Plasmid

#61937

Purpose

Expression of hUb in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

human ubiquitin (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-SznE

Plasmid

#139282

Purpose

expresses SznE in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

sznE

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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