We narrowed to 7,531 results for: ski

1. pRS414 Hsh155 K335N

   Plasmid

   #87274

   Purpose

   Encodes Hsh155 K335N for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   K335N

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS414 Hsh155 H331Q

   Plasmid

   #87272

   Purpose

   Encodes Hsh155 H331Q for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   H331Q

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS414 Hsh155 H331D

   Plasmid

   #87271

   Purpose

   Encodes Hsh155 H331D for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   H331D

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS414 Hsh155 N295D

   Plasmid

   #87270

   Purpose

   Encodes Hsh155 N295D for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   N295D

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS414 Hsh155 E291D

   Plasmid

   #87266

   Purpose

   Encodes Hsh155 E291D for expression in yeast

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

   Insert

   HSH155 (HSH155 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   E291D

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCellFree_G03 RRN3

   Plasmid

   #67112

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RRN3 (RRN3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCellFree_G03 TFAP4

   Plasmid

   #67073

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAP4 (TFAP4 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   P338A

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. L284F Cus2p

    Plasmid

    #73059

    Purpose

    S. cerevisiae Cus2p gene with L284F Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Leucine 284 to Phenylalanine

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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11. D282N Cus2p

    Plasmid

    #73060

    Purpose

    S. cerevisiae Cus2p gene with D282N Mutation

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 Dec 2.

    Insert

    CUS2p (CUS2 Budding Yeast)
    

    Tags

    6x His tag fused to TEV cleavage site

    Expression

    Bacterial and Yeast

    Mutation

    Asp 282 to Asn

    Promoter

    T7

    Availability

    Academic Institutions and Nonprofits only

    

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12. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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13. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-MEG3

    Plasmid

    #44727

    Depositor

    Anne Klibanski

    Article

    Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

    Insert

    Maternally Expressed Gene 3 (MEG3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

    Plasmid

    #126552

    Purpose

    Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    CD8a and GFP

    Mutation

    Delta gag/pol

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

    Plasmid

    #126553

    Purpose

    Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    Thy1.2 and GFP

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Tags

    Hexa his

    Expression

    Insect

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-dCas9VP192-T2A-EGFP-shP53

    Plasmid

    #69535

    Purpose

    Episomal plasmid encoding dCas9VP192 and p53 shRNA

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Insert

    dCas9-dCas9VP192-GFP-shp53
    

    Use

    CRISPR

    Tags

    EGFP

    Promoter

    CAG

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-LacI

    Plasmid

    #183920

    Purpose

    Lac repressor labeled with GFP; can be used to label lacO sites

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    GFP-LacI (lacI Synthetic)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    SV2

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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