We narrowed to 14,607 results for: pET

1. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LgBiT-ST

   Plasmid

   #207633

   Purpose

   A plasmid encoding LgBiT fused to SpyTag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   LgBiT-SpyTag
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-Tev-UbE1

   Plasmid

   #186804

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE1 (UBA1 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPdX

   Plasmid

   #85084

   Purpose

   Expresses putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 73 to Serine and Cysteine 85 to …

   Promoter

   T7 promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1E2D1

   Plasmid

   #161606

   Purpose

   Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009 . 464():211-31.

   Insert

   MSP1E2D1
   

   Use

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSP1E3D1_D73C

   Plasmid

   #39328

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009 . 464():211-31.

   Insert

   MSP1E3D1_D73C (APOA1 synthetic gene)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   cys mutant of MSP1E3D1

   Promoter

   T7

   Available since

   Sept. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. His-Tev-UbE2D3

   Plasmid

   #186805

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-UbE2D3 (UBE2D3 Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJ806

   Plasmid

   #39312

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009 . 464():211-31.

    Insert

    MSP1E1D1
    

    Use

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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