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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,399 results for: pho;

Showing: 9381 - 9399 of 9399 results

  1. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid
    #210021
    Purpose
    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.
    Depositor
    Vesa Hytönen
    Article
    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459
    Insert
    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Synthetic, Human)
    Tags
    MycTag, CTag
    Expression
    Mammalian
    Mutation
    Transferrin receptor transmembrane domain with Y2…
    Promoter
    CMV promoter
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    210021-plasmid-map-sequence-id-425593

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  7. Desmoplakin II acceptor only control (F40-based tension sensor)

    Plasmid
    #118719
    Purpose
    The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)(Y67G)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118719-plasmid-map-sequence-id-230445

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  8. PF3D7_0912400-COMP-blac-flag-his

    Plasmid
    #110966
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    alkaline phosphatase, putative (PF3D7_0912400 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110966-plasmid-map-sequence-id-224238

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  10. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131007-plasmid-map-sequence-id-305185

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  11. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid
    #131006
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    V5 - 10 total of V5 epitope tag
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131006-plasmid-map-sequence-id-305181

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  12. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid
    #130987
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-Myc WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    Myc - 10 total Myc tags
    Mutation
    "dark" variant with GGG fluorophore ver…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130987-plasmid-map-sequence-id-305000

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  14. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid
    #131005
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-FLAG WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    FLAG - 10 total FLAG tags
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131005-plasmid-map-sequence-id-305005

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Showing: 9381 - 9399 of 9399 results