We narrowed to 15,910 results for: grna

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pU6-1 (GB1204)

Plasmid

#75405

Purpose

Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

AtU6-1 promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMEL15_MATz

Plasmid

#166099

Purpose

Expression of gRNA targeting the MAT locus

Depositor

Rebecca Burgess

Insert

MAT Z1

Use

CRISPR

Expression

Yeast

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHACK-QF2

Plasmid

#80274

Purpose

QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs

Depositor

Christopher Potter

Publication

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP

Plasmid

#196989

Purpose

Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNA

Depositor

Elizabeth Kirby

Publication

Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

Insert

Non-Target

Use

CRISPR and Lentiviral

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Publication

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

Plasmid

#177235

Purpose

Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgMark2/sgMark3/sgMark4

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Publication

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Expression

Mammalian

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pROS15_MATz

Plasmid

#166100

Purpose

Expression of dual gRNA targeting the MAT locus

Depositor

Rebecca Burgess

Insert

MAT Z1

Use

CRISPR

Expression

Yeast

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPN10-gDM1d

Plasmid

#114387

Purpose

pPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGG

Depositor

Vincent Dion

Publication

Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

Insert

gDM1d

Use

CRISPR

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-FnCpf1

Plasmid

#129554

Purpose

Contains codon-optimized FnCpf1 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Publication

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

FnCpf1

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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SaCas9v2-Puro

Plasmid

#178802

Purpose

SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_sgGFP3

Plasmid

#119875

Purpose

gRNA expression vector

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGB 35s:dCas:VP64:tNos (GB1189)

Plasmid

#75403

Purpose

Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional Activator

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

dCas:VP64

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

35S

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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PX458 eSp(1.1) V3

Plasmid

#226963

Purpose

CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLIII CKBs CRISPR with guides at 1-2 and 2-3

Plasmid

#238525

Purpose

Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha3

Depositor

Michael Hothorn

Insert

AtCK2alpha3 (AT2G23080 Mustard Weed)

Use

CRISPR

Expression

Plant

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLII-CRISPR for CKA4

Plasmid

#238521

Purpose

Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4

Depositor

Michael Hothorn

Insert

AtCK2alpha4 (AT2G23070 Mustard Weed)

Use

CRISPR

Expression

Plant

Available since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR

Plasmid

#189943

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-tagBFP

Depositor

Kenneth Yamada

Publication

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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